In vivo cell tracking by bioluminescence imaging after transplantation of bioengineered cell sheets to the knee joint

Biomaterials
Yuko TakakuMasato Sato

Abstract

In our previous studies, we have demonstrated effective regeneration of cartilage through the creation and application of layered cell sheets that combine both chondrocytes and synovial cells. In this study, we were able to demonstrate that cells derived from cell sheets can survive for long periods after transplantation into rat knee joints having osteochondral defects. We established a method for generating cell sheets from firefly luciferase-expressing chondrocytes obtained from transgenic Lewis rats, and carried out allogenic transplantation of these cell sheets into wild-type Lewis rats. We then administered luciferin and monitored the survival of the transplanted cells by using bioluminescence imaging (BLI). Our data showed that the transplanted cells survived and could be detected for more than 21 months, which was longer than expected. Furthermore, the BLI data showed that the transplanted cells remained in the knee joint and did not migrate to other parts of the body, thus confirming the safety of the cell sheets. In this study, we monitored the duration of survival of cell sheets composed of only chondrocytes, only synovial cells, or both chondrocytes and synovial cells, and found that all three types of cell sheets s...Continue Reading

References

May 5, 1997·FEBS Letters·M OkabeY Nishimune
Dec 16, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·L A SolchagaA I Caplan
Aug 25, 2001·Biochemical and Biophysical Research Communications·Y HakamataE Kobayashi
Oct 30, 2001·Osteoarthritis and Cartilage·P Mainil-VarletR P Jakob
Jun 5, 2002·The Journal of Bone and Joint Surgery. British Volume·M OchiJ Iwasa
Feb 22, 2005·Biochemical and Biophysical Research Communications·Hirokazu InoueEiji Kobayashi
Jul 19, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Kazunori MasuokaKoichi Nemoto
Apr 28, 2006·Transplantation·Yoji HakamataEiji Kobayashi
Sep 5, 2006·Biochemical and Biophysical Research Communications·Nagatoshi KaneshiroJoji Mochida
May 21, 2008·Tissue Engineering. Part a·Toshihiro NagaiJoji Mochida
Jun 12, 2008·Medical & Biological Engineering & Computing·Masato SatoJoji Mochida
Mar 10, 2009·BMC Biotechnology·Genya MitaniJoji Mochida
Jan 31, 2013·Nature Communications·Hidekazu SekineTeruo Okano
Jul 23, 2013·Journal of Tissue Engineering and Regenerative Medicine·Mami KokuboJoji Mochida

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Citations

Jun 23, 2015·Journal of Biomedical Materials Research. Part a·Akiyuki HasegawaTeruo Okano
Nov 13, 2015·Tissue Engineering. Part C, Methods·Miyuki YokoyamaJoji Mochida
Nov 15, 2014·Tissue Engineering. Part a·Ryo ShimizuMitsuo Ochi
Feb 16, 2017·Journal of Tissue Engineering and Regenerative Medicine·Yoshiki TaniJoji Mochida
May 5, 2017·Tissue Engineering. Part C, Methods·Seiju HayashiMitsuo Ochi
Jul 31, 2018·Journal of Tissue Engineering and Regenerative Medicine·Takumi TakahashiMasahiko Watanabe
Aug 4, 2018·Journal of Tissue Engineering and Regenerative Medicine·Yoshiki TaniJoji Mochida
Jan 17, 2019·Tissue Engineering. Part C, Methods·Claire CrowleyLuca Urbani
Feb 8, 2019·Tissue Engineering. Part C, Methods·Rodolfo E De la VegaRyan M Porter
Oct 17, 2014·Annals of Biomedical Engineering·Jordan E TrachtenbergAntonios G Mikos
Jul 29, 2017·Advanced Functional Materials·Forrest GoodfellowQun Zhao
May 22, 2019·International Journal of Oral Science·Yuezhi LuXinquan Jiang
Jul 12, 2020·Journal of Tissue Engineering and Regenerative Medicine·Daichi TakizawaMasahiko Watanabe
Nov 25, 2020·Emerging Topics in Life Sciences·Makoto KondoTeruo Okano

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