In vivo cellular imaging of magnetically labeled hybridomas in the spleen with a 1.5-T clinical MRI system

Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine
P SmirnovO Clément

Abstract

The feasibility of in vivo cellular imaging using a 1.5 T clinical magnet was studied in the mouse. Hybridoma cells were labeled with anionic gamma-Fe2O3 superparamagnetic iron oxide nanoparticles. These were internalized by the endocytose pathway. Both electron spin resonance and magnetophoresis as a measure of the labeled cells migration velocity under a magnetic field were used to quantify particle uptake. A fast (< 2 hr) and substantial (up to 5 pg of iron per cell) internalization of nanoparticles by hybridomas was found, with good agreement between the two methods used. Hybridomas labeled with 2.5 pg iron per cell were injected intraperitoneally to male Swiss nude mice. A decrease in the spleen signal, suggesting a "homing" of labeled hybridomas to this organ, was found 24 hr later by MRI performed at 1.5 T. Furthermore, in labeled cells recovered from the spleen by ex vivo magnetic sorting, a mean of 0.5 pg iron per cell was found, i.e., a value five times lower than that of the injected hybridomas. This finding is consistent with in vivo proliferation of these cells. In addition, the amount of labeled hybridomas present in the spleen was found to correlate with MRI signal intensity.

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Citations

Mar 6, 2007·The European Physical Journal. E, Soft Matter·C RivièreR Perzynski
Apr 12, 2011·The European Physical Journal. E, Soft Matter·P Domínguez-GarcíaM A Rubio
Nov 29, 2008·Journal of Translational Medicine·Reinhard MeierHeike E Daldrup-Link
May 6, 2008·Biomaterials·Claire Wilhelm, Florence Gazeau
Jul 1, 2008·Nuclear Medicine and Biology·Reinhard MeierHeike E Daldrup-Link
May 4, 2013·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Jung Hwan HwangChul-Ho Lee
Feb 3, 2009·Journal of Colloid and Interface Science·P Domínguez-GarcíaM A Rubio
Dec 29, 2006·Contrast Media & Molecular Imaging·P SmirnovB Gillet
Aug 10, 2006·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Pierre SmirnovOlivier Clément
Sep 14, 2006·Journal of Magnetic Resonance Imaging : JMRI·Yusuke InoueKuni Ohtomo
Oct 6, 2005·NMR in Biomedicine·Brian J NiemanR Mark Henkelman
Nov 9, 2005·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·De-En HuKevin M Brindle
Feb 2, 2006·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Enzo TerrenoSilvio Aime
Apr 4, 2007·Nanotechnology·Ingrid HilgerWerner A Kaiser
Feb 20, 2013·Biologie aujourd'hui·Claire Wilhelm, Florence Gazeau
May 28, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Mei-Ling ChenJian-Hua Wang
Oct 2, 2009·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·P Domínguez-GarcíaMiguel A Rubio
Feb 1, 2008·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·P Domínguez-GarcíaM A Rubio

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