In vivo characterization of the dTDP-D-desosamine pathway of the megalomicin gene cluster from Micromonospora megalomicea

Microbiology
Eduardo RodríguezHugo Gramajo

Abstract

In vivo reconstitution of the dTDP-D-desosamine pathway of the megalomicin gene cluster from Micromonospora megalomicea was achieved by expression of the genes in Escherichia coli. LC/MS/MS analysis of the dTDP-sugar intermediates produced by operons containing different sets of genes showed that production of dTDP-D-desosamine from dtdp-4-keto-6-deoxy-D-glucose requires only four biosynthetic steps, catalysed by MegCIV, MegCV, MegDII and MegDIII, and that MegCII is not involved. Instead, bioconversion studies demonstrated that MegCII is needed together with MegCIII to catalyse transfer of D-desosamine to 3-alpha-mycarosylerythronolide B.

References

May 1, 1990·Journal of Bacteriology·J M WeberJ P DeWitt
Jun 1, 1969·The Journal of Antibiotics·J A WaitzM J Weinstein
Jun 1, 1969·The Journal of Antibiotics·M J WeinsteinJ A Waitz
Feb 16, 1996·The Journal of Biological Chemistry·P BonayB Alarcón
Apr 1, 1997·Natural Product Reports·A C Weymouth-Wilson
Oct 3, 1998·Antimicrobial Agents and Chemotherapy·P BonayA Alcina
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y XueD H Sherman
Aug 5, 2003·Journal of Industrial Microbiology & Biotechnology·Sumati MurliJames T Kealey
Aug 12, 2004·Journal of the American Chemical Society·Ho Young LeeSuzanne Walker
Dec 23, 2004·Journal of the American Chemical Society·Charles E MelançonHung-wen Liu
May 5, 2005·Applied and Environmental Microbiology·Salvador PeirúHugo Gramajo
Sep 28, 2005·Angewandte Chemie·Ping-hui SzuHung-wen Liu
Oct 13, 2005·Journal of the American Chemical Society·Yanqiu YuanSuzanne Walker

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Citations

Apr 4, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·K E van StraatenD A R Sanders
Apr 27, 2010·Applied and Environmental Microbiology·Mariana UseglioHugo Gramajo
Apr 14, 2009·Methods in Enzymology·Salvador PeirúHugo G Menzella
Nov 6, 2013·Proceedings of the National Academy of Sciences of the United States of America·Roland D KerstenBradley S Moore
Dec 3, 2016·International Journal of Systematic and Evolutionary Microbiology·Shanshan ZhaoXiangjing Wang
Sep 4, 2015·Chemical Society Reviews·Dong-Mei LiangJian-Jun Qiao

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