In vivo chemical and structural analysis of plant cuticular waxes using stimulated Raman scattering microscopy

Plant Physiology
George R LittlejohnJulian Moger

Abstract

The cuticle is a ubiquitous, predominantly waxy layer on the aerial parts of higher plants that fulfils a number of essential physiological roles, including regulating evapotranspiration, light reflection, and heat tolerance, control of development, and providing an essential barrier between the organism and environmental agents such as chemicals or some pathogens. The structure and composition of the cuticle are closely associated but are typically investigated separately using a combination of structural imaging and biochemical analysis of extracted waxes. Recently, techniques that combine stain-free imaging and biochemical analysis, including Fourier transform infrared spectroscopy microscopy and coherent anti-Stokes Raman spectroscopy microscopy, have been used to investigate the cuticle, but the detection sensitivity is severely limited by the background signals from plant pigments. We present a new method for label-free, in vivo structural and biochemical analysis of plant cuticles based on stimulated Raman scattering (SRS) microscopy. As a proof of principle, we used SRS microscopy to analyze the cuticles from a variety of plants at different times in development. We demonstrate that the SRS virtually eliminates the back...Continue Reading

Citations

Mar 15, 2016·Frontiers in Chemistry·Batirtze Prats MateuNotburga Gierlinger
Mar 11, 2016·Nature Protocols·Holly J ButlerFrancis L Martin
May 5, 2016·Journal of Microscopy·Mark D FrickerMichael J Deeks
Sep 17, 2016·Science·Eleanore T Wurtzel, Toni M Kutchan
Oct 17, 2017·Journal of Experimental Botany·Johann PetitChristophe Rothan
May 3, 2016·Chemistry & Biodiversity·Na GuoYanjun Guo
Feb 10, 2021·Plant Methods·Nadia SasaniNotburga Gierlinger
Apr 29, 2019·Plant Physiology and Biochemistry : PPB·Maree BrennanCairistiona F E Topp
Aug 31, 2021·RSC Chemical Biology·Keiko MidorikawaKeiji Numata

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