Jun 15, 2010

In vivo chromatin organization of mouse rod photoreceptors correlates with histone modifications

PloS One
Caroline KizilyaprakP Schultz

Abstract

The folding of genetic information into chromatin plays important regulatory roles in many nuclear processes and particularly in gene transcription. Post translational histone modifications are associated with specific chromatin condensation states and with distinct transcriptional activities. The peculiar chromatin organization of rod photoreceptor nuclei, with a large central domain of condensed chromatin surrounded by a thin border of extended chromatin was used as a model to correlate in vivo chromatin structure, histone modifications and transcriptional activity. We investigated the functional relationships between chromatin compaction, distribution of histone modifications and location of RNA polymerase II in intact murine rod photoreceptors using cryo-preparation methods, electron tomography and immunogold labeling. Our results show that the characteristic central heterochromatin of rod nuclei is organized into concentric domains characterized by a progressive loosening of the chromatin architecture from inside towards outside and by specific combinations of silencing histone marks. The peripheral heterochromatin is formed by closely packed 30 nm fibers as revealed by a characteristic optical diffraction signal. Unexpect...Continue Reading

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  • Citations36

Citations

Mentioned in this Paper

Electron Microscope Tomography
Photoreceptors
RNA Polymerase II
Immunofluorescence Assay
Nucleosomes
Histone antigen
Liquid Crystals
Hyrex Brand of Dimenhydrinate
Carboxy-Terminal Amino Acid
Protein Methylation

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