In Vivo Chronic Stimulation Unveils Autoreactive Potential of Wiskott-Aldrich Syndrome Protein-Deficient B Cells.

Frontiers in Immunology
Maria Carmina CastielloMarita Bosticardo

Abstract

Wiskott-Aldrich syndrome (WAS) is a primary immunodeficiency caused by mutations in the gene encoding the hematopoietic-specific WAS protein (WASp). WAS is frequently associated with autoimmunity, indicating a critical role of WASp in maintenance of tolerance. The role of B cells in the induction of autoreactive immune responses in WAS has been investigated in several settings, but the mechanisms leading to the development of autoimmune manifestations have been difficult to evaluate in the mouse models of the disease that do not spontaneously develop autoimmunity. We performed an extensive characterization of Was-/- mice that provided evidence of the potential alteration in B cell selection, because of the presence of autoantibodies against double-stranded DNA, platelets, and tissue antigens. To uncover the mechanisms leading to the activation of the potentially autoreactive B cells in Was-/- mice, we performed in vivo chronic stimulations with toll-like receptors agonists (LPS and CpG) and apoptotic cells or infection with lymphocytic choriomeningitis virus. All treatments led to increased production of autoantibodies, increased proteinuria, and kidney tissue damage in Was-/- mice. These findings demonstrate that a lower clear...Continue Reading

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Citations

Jul 30, 2020·JCI Insight·Giulia Maria PipernoFederica Benvenuti
Mar 22, 2019·Frontiers in Immunology·Minghui He, Lisa S Westerberg
Aug 17, 2021·The National Medical Journal of India·Behnam SoboutiNima Rezaei

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Methods Mentioned

BETA
flow cytometry
FACS
ELISA
Assay

Software Mentioned

FlowJo

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