In vivo clearance of surfactant lipids during acute pulmonary inflammation

Respiratory Research
Jaret L Malloy, Jo Rae Wright

Abstract

A decrease in pulmonary surfactant has been suggested to contribute to the lung dysfunction associated with pulmonary inflammation. A number of studies have implicated surfactant clearance as a possible mechanism for altered pool sizes. The objective of the current study was to specifically investigate the mechanisms of surfactant clearance in a rodent model of acute pulmonary inflammation. Inflammation was induced by intrapulmonary instillation of lipopolysaccharide (LPS: 100 microg/kg). Lipid clearance was assessed at 18 and 72 hours post-LPS instillation by intratracheal administration of radiolabel surfactant-like liposomes 2 hours prior to isolation and analysis of inflammatory cells and type II cells. At both 18 and 72 hours after LPS instillation there was significantly less radioactivity recovered in the lavage fluid compared to respective control groups (p < 0.05). At both time points, the number of cells recovered by lavage and their associated radioactivity was greater compared to control groups (p < 0.01). There was no difference in recovery of radioactivity by isolated type II cells or other cells obtained from enzymatic digestion of lung tissue. These results show that increased clearance of surfactant lipids in o...Continue Reading

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Citations

Jan 24, 2007·Physiological Reviews·Kendra J GreenleeFarrah Kheradmand
Apr 21, 2007·Journal of Applied Physiology·Amy ForbesRuud Veldhuizen
Sep 22, 2010·The Journal of Surgical Research·Ioanna VasileiouGeorgia Kostopanagiotou

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Methods Mentioned

BETA
lavages
bronchoalveolar lavage
lavage
bronchoalveolar
confocal microscopy

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