PMID: 8596672Oct 1, 1995Paper

In vivo cleavage of immunoglobulin A1 by immunoglobulin A1 proteases from Prevotella and Capnocytophaga species

Oral Microbiology and Immunology
E V FrandsenM Kilian

Abstract

Immunoglobulin A1 (IgA1) proteases secreted by oral Prevotella and Capnocytophaga species specifically cleave IgA1 at the same peptide bond in the hinge region, leaving intact monomeric Fab and Fc fragments. Assuming that Prevotella- and Capnocytophaga-induced Fab fragments of IgA1 expose a specific immunogenic neoepitope at the cleavage site, we established an enzyme-linked immunosorbent assay to measure human serum antibodies to this neoepitope as indirect evidence of in vivo activity of Prevotella and Capnocytophaga IgA1 proteases. The assay used a monoclonal antibody with specificity for the neoepitope, and the ability to block binding of the monoclonal antibody to the neoepitope was investigated. Absorption of sera with Prevotella melaninogenica-induced Fab fragments of IgA1 resulted in removal of antibodies blocking binding of the monoclonal antibody, whereas absorption with Fab fragments induced by bacterial IgA1 proteases of other cleavage specificities did not remove blocking antibodies. Consequently, we assume that the antibodies detected had been induced by a neoepitope an the Fab fragment of IgA1 exposed exclusively after cleavage with IgA1 proteases from Prevotella and Capnocytophaga, indicating in vivo activity of...Continue Reading

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Citations

Dec 1, 1996·FEMS Microbiology Letters·K R Alugupalli, S Kalfas
Aug 12, 2014·The Lancet Global Health·Denise Baratti-MayerUNKNOWN Geneva Study Group on Noma (GESNOMA)
Apr 8, 1999·Clinical and Experimental Immunology·D F KinaneA Buckley
May 1, 1996·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·M KilianE V Frandsen
Oct 3, 2013·PLoS Neglected Tropical Diseases·Antoine HuygheUNKNOWN Geneva Study Group on Noma
Aug 4, 2015·Revue de stomatologie, de chirurgie maxillo-faciale et de chirurgie orale·N ZwetyengaA Emparanza
Feb 24, 2001·Journal of Endodontics·R NissanR Stevens
Sep 5, 2018·European Journal of Oral Sciences·Mogens Kilian
Jul 23, 1998·Critical Reviews in Biochemistry and Molecular Biology·P Van den SteenG Opdenakker
Jul 1, 1997·Clinical and Diagnostic Laboratory Immunology·E V FrandsenM Kilian

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