In Vivo Consumption of Cranberry Exerts ex Vivo Antiadhesive Activity against FimH-Dominated Uropathogenic Escherichia coli: A Combined in Vivo, ex Vivo, and in Vitro Study of an Extract from Vaccinium macrocarpon

Journal of Agricultural and Food Chemistry
Nasli RafsanjanyAndreas Hensel

Abstract

For investigation of the molecular interaction of cranberry extract with adhesins of uropathogenic Escherichia coli (UPEC), urine from four volunteers consuming standardized cranberry extract (proanthocyanidin content = 1.24%) was analyzed within ex vivo experiments, indicating time-dependent significant inhibition of 40-50% of bacterial adhesion of UPEC strain NU14 to human T24 bladder cells. Under in vitro conditions a dose-dependent increase in bacterial adhesion was observed with proanthocyanidin-enriched cranberry Vaccinium macrocarpon extract (proanthocyanidin content = 21%). Confocal laser scanning microscopy and scanning electron microscopy proved that V.m. extract led to the formation of bacterial clusters on the outer plasma membrane of the host cells without subsequent internalization. This agglomerating activity was not observed when a PAC-depleted extract (V.m. extract(≠PAC)) was used, which showed significant inhibition of bacterial adhesion in cases where type 1 fimbriae dominated and mannose-sensitive UPEC strain NU14 was used. V.m. extract(≠PAC) had no inhibitory activity against P- and F1C-fimbriae dominated strain 2980. Quantitative gene expression analysis indicated that PAC-containing as well as PAC-deplete...Continue Reading

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May 21, 2016·Frontiers in Microbiology·Sylvie MiquelChristiane Forestier
Jun 9, 2016·International Journal of Experimental Pathology·Karellen Beren García MéndezAnne Keriel
Jan 1, 2019·Critical Reviews in Food Science and Nutrition·Bárbara Nieva-EchevarríaMaría D Guillén
Jul 28, 2018·Journal of the Pediatric Infectious Diseases Society·Anum KhanMehreen Arshad
Jul 16, 2020·Antibiotics·Meysam SarsharDaniela Scribano
Jul 29, 2020·Frontiers in Microbiology·Paul LoubetAlbert Sotto
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Jan 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mutaib M MashraqiSyed Mohd Danish Rizvi
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Dec 31, 2020·Antibiotics·Oghenekome A GbinigieChristopher C Butler
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