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In vivo CRISPR screening in CD8 T cells with AAV-Sleeping Beauty hybrid vectors identifies membrane targets for improving immunotherapy for glioblastoma

Nature Biotechnology

Nov 23, 2019

Lupeng YeSidi Chen

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Abstract

Targeting membrane proteins could improve the efficacy of T cell-based immunotherapies. To facilitate the identification of T cell targets, we developed a hybrid genetic screening system where the Sleeping Beauty (SB) transposon and single guide RNA cassette are nested in an adeno-assoc...read more

Mentioned in this Paper

Dependovirus
Genetic Engineering
Genetic Vectors
Glioblastoma
Immunotherapy
Membrane Proteins
Laboratory mice
Culicidae
Melanocytic Nevus
RNA
112
5
98
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In vivo CRISPR screening in CD8 T cells with AAV-Sleeping Beauty hybrid vectors identifies membrane targets for improving immunotherapy for glioblastoma

Nature Biotechnology

Nov 23, 2019

Lupeng YeSidi Chen

PMID: 31548728

DOI: 10.1038/s41587-019-0246-4

Abstract

Targeting membrane proteins could improve the efficacy of T cell-based immunotherapies. To facilitate the identification of T cell targets, we developed a hybrid genetic screening system where the Sleeping Beauty (SB) transposon and single guide RNA cassette are nested in an adeno-assoc...read more

Mentioned in this Paper

Dependovirus
Genetic Engineering
Genetic Vectors
Glioblastoma
Immunotherapy
112
5
98

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