In Vivo Dark-Field Radiography for Early Diagnosis and Staging of Pulmonary Emphysema

Investigative Radiology
Katharina HellbachFranz Pfeiffer

Abstract

The aim of this study was to evaluate the suitability of in vivo x-ray dark-field radiography for early-stage diagnosis of pulmonary emphysema in mice. Furthermore, we aimed to analyze how the dark-field signal correlates with morphological changes of lung architecture at distinct stages of emphysema. Female 8- to 10-week-old C57Bl/6N mice were used throughout all experiments. Pulmonary emphysema was induced by orotracheal injection of porcine pancreatic elastase (80-U/kg body weight) (n = 30). Control mice (n = 11) received orotracheal injection of phosphate-buffered saline. To monitor the temporal patterns of emphysema development over time, the mice were imaged 7, 14, or 21 days after the application of elastase or phosphate-buffered saline. X-ray transmission and dark-field images were acquired with a prototype grating-based small-animal scanner. In vivo pulmonary function tests were performed before killing the animals. In addition, lungs were obtained for detailed histopathological analysis, including mean cord length (MCL) quantification as a parameter for the assessment of emphysema. Three blinded readers, all of them experienced radiologists and familiar with dark-field imaging, were asked to grade the severity of emph...Continue Reading

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Citations

Jan 3, 2016·The Review of Scientific Instruments·Mark MüllerFranz Pfeiffer
Dec 2, 2015·Scientific Reports·Andre YaroshenkoFranz Pfeiffer
Dec 3, 2015·EBioMedicine·A VelroyenF Pfeiffer
Feb 9, 2018·Investigative Radiology·Katharina HellbachFelix G Meinel
Aug 18, 2018·Physics in Medicine and Biology·Maria SeifertGisela Anton
Sep 28, 2018·PloS One·Konstantin WillerPeter B Noël
Sep 13, 2017·Journal of Medical Imaging·Sebastian KaepplerChristian Riess
Feb 3, 2018·Scientific Reports·Katharina HellbachAli Ö Yildirim
Feb 10, 2018·Scientific Reports·Katharina HellbachMaximilian F Reiser
Feb 8, 2019·Physics in Medicine and Biology·Veronika LudwigGisela Anton
Jul 12, 2019·European Radiology Experimental·Alexander A FingerleFranz Pfeiffer
Sep 8, 2016·Investigative Radiology·Katharina HellbachFelix G Meinel
Apr 7, 2020·Investigative Radiology·Eva-Maria BraigDaniela Pfeiffer
May 28, 2020·Physics in Medicine and Biology·Eva-Maria BraigJulia Herzen
Nov 19, 2015·Physics in Medicine and Biology·Hildur EinarsdóttirFranz Pfeiffer
Mar 19, 2016·Physical Review Letters·Matias KagiasMarco Stampanoni
Nov 20, 2016·Optics Express·M MarschnerF Pfeiffer
Jun 20, 2019·Scientific Reports·Andreas P SauterFranz Pfeiffer
Jun 27, 2019·Scientific Reports·Lina FelsnerChristian Riess
Mar 28, 2017·Scientific Reports·Kai SchererJulia Herzen
Aug 9, 2017·Scientific Reports·Maximilian von TeuffenbachPeter B Noël
Sep 15, 2020·Biomedical Optics Express·Marcus J KitchenStuart B Hooper
Mar 25, 2017·Scientific Reports·Katharina HellbachFelix G Meinel
Jul 8, 2017·Scientific Reports·Lukas B GromannJulia Herzen
Mar 14, 2019·Scientific Reports·Maria SeifertGisela Anton
Oct 24, 2018·International Journal of Computer Assisted Radiology and Surgery·Johannes BoppChristian Riess
Jan 27, 2021·European Radiology Experimental·Jana AndrejewskiDaniela Pfeiffer
Jul 28, 2021·Medical Physics·Manuela FrankFranz Pfeiffer

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