In vivo delivery of heat shock protein 70 accelerates wound healing by up-regulating macrophage-mediated phagocytosis

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Joseph T KovalchinRajiv Y Chandawarkar

Abstract

Injury causes tissue breakdown, which releases large quantities of intracellular contents into the extracellular space. Some of these materials are well-established activators of the immune system and include heat shock proteins (HSPs), uric acid, nucleotides, High Mobility Group Box-1 protein (HMGB-1), and DNA. Here, we show that in vivo delivery of HSPs into BALB/cJ mice with full-thickness wounds accelerates the rate of wound closure by 60% as compared with control-treated mice. The onset is rapid and the effect is sustained, dose dependent, and protein specific. Adoptive transfer of RAW264 macrophages pretreated with HSP70 into naïve recipients with a wound transfers the HSP-mediated effect on the rate of wound closure. Further, we demonstrate that part of the mechanism by which HSP70 accelerates wound closure is through the stimulation of macrophage-mediated phagocytosis of wound debris. Disabling the HSP70-mediated enhancement of phagocytosis abrogates the HSP-mediated acceleration of the healing process. These findings create two opportunities: one, therapeutic, wherein HSP70 could be used in the clinical management of wounds; and two, pathophysiologic, to decode signals by which the host defenses recognize and respond t...Continue Reading

References

Dec 1, 1991·Current Opinion in Cell Biology·W J Welch
Jan 2, 1992·Nature·M J Gething, J Sambrook
Sep 25, 1995·Biochemical and Biophysical Research Communications·M OberringerR G Hanselmann
Jun 1, 1995·British Journal of Plastic Surgery·S E CrossT C Teo
May 1, 1993·Scientific American·W J Welch
Mar 26, 1998·Biochemical and Biophysical Research Communications·I YamamuraK Nagata
Mar 26, 1998·Biochemical and Biophysical Research Communications·A ShuklaB S Srivastava
Aug 28, 1998·European Journal of Pharmacology·C R Byrne, P J Hanson
Oct 17, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A F LaplanteL Germain
Apr 30, 1999·Physiological Reviews·A L Fink
Sep 11, 1999·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·C R RolandM W Flye
Dec 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y ShiK L Rock
Feb 24, 2001·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·R H LeeA Barbul
Mar 15, 2001·Nature Immunology·R J BinderP K Srivastava
Apr 21, 2001·Cardiovascular Drug Reviews·L C AddingL E Gustafsson
Aug 4, 2001·The American Journal of Pathology·Q E LowL A DiPietro
Jan 10, 2002·Critical Care Medicine·Elisabeth S ChristiansIvor J Benjamin
Mar 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Naveed N PanjwaniPramod K Srivastava
Mar 19, 2002·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·J N KeagleD M Young
Mar 27, 2002·Nature Reviews. Immunology·Pramod Srivastava
Jun 28, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ryoichi MoriNaofumi Mukaida
Oct 2, 2002·Immunity·Yves DelnestePascale Jeannin
Nov 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Karen BethkeMichael Heike
Dec 7, 2002·Current Topics in Microbiology and Immunology·R M VabulasH Schild
Apr 23, 2004·Infection and Immunity·Oliver GoldmannEva Medina
Jul 28, 2004·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·H Kondo, S Watabe
Sep 7, 2004·Journal of Materials Science. Materials in Medicine·B KlosterhalfenR S Bhardwaj
Dec 23, 2004·Chinese Journal of Digestive Diseases·Elaine O PetrofEugene B Chang
Feb 9, 2005·The Journal of Veterinary Medical Science·Jae-sung BaeHee Kyung Jin

❮ Previous
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Citations

Feb 15, 2008·Langenbeck's Archives of Surgery·Derek ZiekerStefan Beckert
Jul 16, 2008·Cell Stress & Chaperones·Bindi M DoshiLawrence E Hightower
Dec 7, 2010·Cell Stress & Chaperones·Matthew B Fortes, Martin Whitham
Dec 18, 2009·Nature Immunology·Alexander V Chervonsky
Mar 26, 2009·Plastic and Reconstructive Surgery·Aleksandra KrajewskiRajiv Y Chandawarkar
Jul 31, 2009·Journal of Biomechanical Engineering·Alfred S Song, Kenneth R Diller
Apr 10, 2009·American Journal of Physiology. Cell Physiology·Hiroe ShiratsuchiMarc D Basson
Feb 23, 2007·Journal of Translational Medicine·Kavita DeonarineDavid F Stroncek
Nov 22, 2011·Head & Face Medicine·Majeed RanaNils-Claudius Gellrich
Jan 6, 2011·Journal of Oral Science·Mayra T VasquesLuciana Corrêa
Apr 2, 2014·Journal of Neuroinflammation·Debbie A E HendrickxInge Huitinga
Apr 15, 2014·BMC Complementary and Alternative Medicine·Marcus GadauShi-Ping Zhang
Nov 19, 2011·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Autumn M StarnesJorge Garcia-Zuazaga
Mar 30, 2007·Digestive Diseases and Sciences·Masaru OdashimaSumio Watanabe
Dec 12, 2012·Journal of Photochemistry and Photobiology. B, Biology·Mayra Torres VasquesLuciana Corrêa
Jul 31, 2012·Veterinary Immunology and Immunopathology·Sarah A Moore, Michael J Oglesbee
Jun 26, 2010·Journal of the American Academy of Dermatology·Stephan SchremlPhilipp Babilas
Dec 10, 2008·Lasers in Surgery and Medicine·Josh T BeckhamE Duco Jansen
Mar 6, 2013·Journal of Cellular Physiology·Esther GiraldoEduardo Ortega
Jul 20, 2010·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·S D DamsF P T Baaijens
Jan 22, 2011·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·S D DamsF P T Baaijens
Mar 5, 2011·Cell Biochemistry and Function·Thiago Gomes HeckPaulo I Homem de Bittencourt
Jan 20, 2011·Lasers in Surgery and Medicine·Josh T BeckhamE Duco Jansen
Aug 30, 2008·The American Journal of Pathology·Lillian B NanneyLeslie I Gold
Sep 21, 2015·Biogerontology·I Martínez de Toda, M De la Fuente
Aug 28, 2015·Cell Stress & Chaperones·Fangfang ChengDezeng Liu
Aug 13, 2015·Zoology : Analysis of Complex Systems, ZACS·Karen L SweazeaCatherine Jarrett
Dec 3, 2014·Fish & Shellfish Immunology·Mirella VazzanaMonica Celi
Jun 24, 2014·Seminars in Immunology·Kate WicksKimberly A Mace
Jan 26, 2017·Acta Biomaterialia·Ziad JulierMikaël M Martino
May 5, 2017·International Journal of Molecular Sciences·Tiago Góss SantosGlaucia Noeli Maroso Hajj
Dec 6, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Savant S ThakurGordon S Lynch
Feb 6, 2019·International Journal of Molecular Sciences·Erminia MarianiLia Pulsatelli
Jan 13, 2009·The Biochemical Journal·Marek MichalakMichal Opas
Sep 1, 2020·Facial Plastic Surgery : FPS·Richard D Gentile
Nov 6, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tawanda ZiningaAddmore Shonhai

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