In vivo detection of 13C isotopomer turnover in the human brain by sequential infusion of 13C labeled substrates.

Journal of Magnetic Resonance
Shizhe LiJun Shen

Abstract

This study demonstrates the feasibility of simultaneously detecting human brain metabolites labeled by two substrates infused in a sequential order. In vivo (13)C spectra of carboxylic/amide carbons were acquired only during the infusion of the second substrate. This approach allowed dynamic detection of (13)C labeling from two substrates with considerably different labeling patterns. [2-(13)C]glucose and [U-(13)C(6)]glucose were used to generate singlet and doublet signals of the same carboxylic/amide carbon atom, respectively. Because of the large one-bond (13)C-(13)C homonuclear J coupling between a carboxylic/amide carbon and an aliphatic carbon (~50 Hz), the singlet and doublet signals of the same carboxylic/amide carbon were well distinguished. The results demonstrated that different (13)C isotopomer patterns could be simultaneously and distinctly measured in vivo in a clinical setting at 3T.

References

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Nov 5, 2005·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Jun Shen
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Jul 25, 2006·Journal of Magnetic Resonance·Su Xu, Jun Shen
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Apr 29, 2015·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Shizhe LiJun Shen

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