PMID: 16514278Mar 4, 2006Paper

In vivo determination of the gap2 gene promoter activity in Giardia lamblia

The Korean Journal of Parasitology
Hye-Won YangSoon-Jung Park

Abstract

A shuttle vector for Escherichia coli and Giardia lamblia was modified to produce a reporter plasmid, which monitors the expression of prescribed gene in G. lamblia by measuring its luciferase activity. Promoter regions of the gap2 gene, one of the genes induced during encystation, were cloned into this plasmid, and the resultant constructs were then transfected into trophozoites of G. lamblia. Transgenic trophozoites containing one of the 3 gap2-luc reporters were induced to encystation, and characterized with respect to gap2 gene expression by measuring their luciferase activities. Giardia containing a gap2-luc fusion of 112-bp upstream region showed full induction of luciferase activity during encystation.

References

Jan 1, 1983·Transactions of the Royal Society of Tropical Medicine and Hygiene·D B Keister
Mar 1, 1995·Molecular Microbiology·M R MowattH H Stibbs
Oct 18, 1996·Molecular and Biochemical Parasitology·X QueF D Gillin
May 9, 1998·Molecular and Biochemical Parasitology·S M SingerT E Nash
Jul 11, 2002·Parasitology Research·Hye-Won YangSoon-Jung Park
May 14, 2003·Molecular and Biochemical Parasitology·H YangS Park

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Citations

May 3, 2014·PLoS Neglected Tropical Diseases·Rishi Kumar NageshanUtpal Tatu
Dec 5, 2020·International Journal for Parasitology·Samantha J Emery-CorbinAaron R Jex

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