In vivo dynamics of the cortical actin network revealed by fast-scanning atomic force microscopy

Microscopy
Yanshu ZhangShige H Yoshimura

Abstract

Together with lamellipodia and stress fibers, a dynamic network of actin filaments in the cell cortex plays a major role in the maintenance of cell morphology and motility. In contrast to lamellipodia, which have been well studied in various motile cells, the dynamics of actin filaments in the cell cortex have not yet been clarified due to a lack of proper imaging techniques. Here, we utilized high-speed atomic force microscopy for live-cell imaging and analyzed cortical actin dynamics in living cells. We successfully measured the polymerization rate and the frequency of filament synthesis in living COS-7 cells, and examined the associated effects of various inhibitors and actin-binding proteins. Actin filaments are synthesized beneath the plasma membrane and eventually descend into the cytoplasm. The inhibitors, cytochalasin B inhibited the polymerization, while jasplakinolide, inhibited the turnover of actin filaments as well as descension of the newly synthesized filaments, suggesting that actin polymerization near the membrane drives turnover of the cortical actin meshwork. We also determined how actin turnover is maintained and regulated by the free G-actin pool and G-actin binding proteins such as profilin and thymosin β4...Continue Reading

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Citations

Oct 28, 2018·Human Brain Mapping·Ana CoitoGijs Plomp
Oct 26, 2018·Frontiers in Cellular Neuroscience·Yolanda Gimenez-MolinaLuis M Gutiérrez
Jul 22, 2018·Journal of Cell Science·Priyamvada Chugh, Ewa K Paluch
May 24, 2021·Biochemical and Biophysical Research Communications·Nur Diyana MaarofShige H Yoshimura

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