In vivo echocardiographic imaging of transplanted human adult stem cells in the myocardium labeled with clinically applicable CliniMACS nanoparticles

Journal of the American Society of Echocardiography : Official Publication of the American Society of Echocardiography
C BaraA Ruhparwar

Abstract

Intramyocardial transplantation of bone marrow-derived cells is currently under clinical evaluation as a therapy of heart failure. A major limitation of all clinical studies dealing with myocardial cell engraftment is the inability to track the fate of the transplanted cells. We present a clinically applicable technique using transesophageal echocardiography (TEE) of CliniMACS nanoparticle labeled transplanted CD133+ cells in ischemic hearts. CD133+ cells were isolated from human bone marrow by CliniMACS magnetic bead selection. Positive (5 x 10(6) cells) cells were transplanted into porcine ischemic myocardium (n = 6). Control animals (n = 5) received medium injection. TEE was performed to demonstrate the distribution of the cells during and 8 weeks after the procedure. Accumulations of labeled CD133+ cells of adjacent injections in the myocardium were discriminatively identified by TEE. TEE is an elegant method for real-time documentation of successful transplantation and cell colony localization of magnetically labeled CD133+ cells in the ischemic myocardium. Our method should be of special interest for TEE-guided transcatheter injection of cells.

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Citations

Mar 1, 2009·Journal of Cardiovascular Translational Research·Andrew M Kahn, Anthony N Demaria
Jul 25, 2008·Nature Clinical Practice. Cardiovascular Medicine·Hung Q LyRoger J Hajjar
Jan 31, 2012·Imaging in Medicine·Yingli FuDara L Kraitchman
Mar 1, 2008·Regenerative Medicine·Ali S Arbab, Joseph A Frank
Aug 6, 2014·Nature Biotechnology·Anna V NaumovaJoseph A Frank
Dec 3, 2014·The Canadian Journal of Cardiology·Rohit Moudgil, Alexander J Dick
Jul 12, 2014·Stem Cells and Development·Dario BongiovanniGiulio Pompilio
Apr 17, 2010·Current Cardiovascular Imaging Reports·Jaehoon Chung, Phillip C Yang
Jun 28, 2011·Journal of the American Society of Echocardiography : Official Publication of the American Society of Echocardiography·Brian D Hoit
Jul 29, 2008·Nanomedicine : Nanotechnology, Biology, and Medicine·L F GamarraE Amaro
Feb 14, 2013·Journal of Cellular and Molecular Medicine·Santiago RouraAntoni Bayes-Genis
Apr 20, 2007·International Journal of Cardiology·Arjang RuhparwarMatthias Karck
Jul 12, 2019·Advanced Drug Delivery Reviews·Colman MooreJesse V Jokerst

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