In vivo effects of morphine on neuronal fate and opioid receptor expression in zebrafish embryos

The European Journal of Neuroscience
F Macho Sanchez-SimonRaquel E Rodriguez

Abstract

Morphine remains one of the most potent analgesic compounds used to control chronic pain despite its known adverse effects. It binds to the opioid receptors mu, delta and kappa, which are involved in aspects of neuronal fate such as cell proliferation, neuroprotection and neuronal differentiation. However, the effect of morphine on these processes is controversial and in vitro studies, as well as in vivo studies on adults and neonates in mammalian models, have not been able to clarify the diverse roles of morphine in the central nervous system. We have used zebrafish embryos to determine in vivo how morphine affects neuronal fate and opioid receptor gene expression and to elucidate if there is a link between these processes. Our results show that at 24 and 48 h post fertilization (hpf) morphine enhances cell proliferation, although it has opposing effects as an inducer of neuronal differentiation at these two stages, increasing the number of certain neuronal populations at 24 hpf and decreasing it at 48 hpf. The present study also demonstrates that in 24-hpf embryos morphine acts as a neuroprotector against glutamate damage in motor neurons and Pax-6-positive neurons. Furthermore, the gene expression of the opioid receptors is ...Continue Reading

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Citations

Jun 19, 2013·Neurotoxicology and Teratology·Héctor CarreñoRosario Arévalo
Apr 18, 2012·Journal of Proteomics·Hui-Qin ZhuoZongwei Cai
Oct 11, 2011·Peptides·Richard J Bodnar
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Aug 1, 2012·Physiological Research·R Rokyta, J Fricová
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Mar 17, 2021·Toxicology and Applied Pharmacology·Krishna Tulasi KirlaThomas Kraemer
Oct 15, 2021·Journal of Applied Toxicology : JAT·Travon CoomanLuis Arroyo

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