In Vivo Effects of Neostigmine and Physostigmine on Neutrophil Functions and Evaluation of Acetylcholinesterase and Butyrylcholinesterase as Inflammatory Markers during Experimental Sepsis in Rats

Mediators of Inflammation
Diane I BitzingerYork A Zausig

Abstract

Recent studies have shown that acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) may serve as important diagnostic and therapeutic targets in sepsis. Since polymorphonuclear neutrophils (PMNs) play a pivotal role in the early phase of sepsis, we evaluated the potential therapeutic effects of cholinesterase inhibitors on PMN functions during cecal ligation and puncture- (CLP-) induced sepsis and investigated the roles of AChE and BChE as inflammatory markers under standardized experimental conditions. Sham surgery or CLP was performed in male Wistar rats (n = 60). Animals were randomized into four groups: physostigmine, 100 μg/kg; neostigmine, 75 μg/kg; 0.9% saline (control group); and sham group, each applied four times over 24 h. The levels of reactive oxygen species (ROS) production and CD11b/CD62l expression were quantified by flow cytometry at t = 0, 6, 15, 20, and 24 h. Blood gas analysis as well as AChE and BChE activity levels was measured by validated point-of-care measurements. Clinical scores and survival times were determined. CLP induced a significant increase in ROS production and CD11b upregulation by rat PMNs. Treatment with physostigmine or neostigmine significantly reduced ROS production and CD11b up...Continue Reading

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Citations

Nov 22, 2020·Journal of Neurochemistry·Christine N Metz, Valentin A Pavlov
Jan 8, 2021·Arhiv za higijenu rada i toksikologiju·Ana Matošević, Anita Bosak
Apr 15, 2021·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Aaron J PolichnowskiDonald B Hoover
Jul 3, 2021·Biomolecules·Laura S KupkeSigrid Karrer

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Methods Mentioned

BETA
Assay
Flow Cytometric
light microscopy

Software Mentioned

G∗Power
SPSS
CellQuest

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