PMID: 9422361Jan 9, 1998Paper

In vivo electrochemical studies of dopamine clearance in the rat substantia nigra: effects of locally applied uptake inhibitors and unilateral 6-hydroxydopamine lesions

Journal of Neurochemistry
A F Hoffman, G A Gerhardt

Abstract

High-speed chronoamperometric recordings were used to measure the uptake and clearance of locally applied dopamine (DA) within the substantia nigra (SN) of anesthetized rats. To establish that DA clearance within the SN was mediated primarily by the DA transporter (DAT) rather than the norepinephrine transporter (NET) or the serotonin transporter (SERT), we locally applied uptake inhibitors with different selectivity profiles for the various amine transporters. Nomifensine, a DAT/NET inhibitor, significantly potentiated both the amplitude and the time course of the DA signals. In contrast, neither the selective NET inhibitor desipramine, nor the selective SERT inhibitor citalopram affected the DA signal, suggesting that NET and SERT do not contribute to DA uptake and clearance within the regions of the SN studied over the concentration ranges (1-5 microM) used. In unilaterally 6-hydroxydopamine-lesioned rats, the time course of the DA signal was increased in both the lesioned SN and striatum, relative to the unlesioned hemisphere, indicating loss of DAT and decreased DA uptake and clearance. In addition, when identical amounts of DA were injected in the striatum and SN, peak signal amplitudes were larger in the SN, suggesting t...Continue Reading

Citations

Sep 14, 2017·Proceedings of the National Academy of Sciences of the United States of America·Poonam ThakurAnders Björklund
Feb 7, 2009·Journal of Neurochemistry·Martin LundbladIngrid Strömberg
Feb 9, 2012·Proceedings of the National Academy of Sciences of the United States of America·Martin LundbladAnders Björklund
Jun 17, 2003·Journal of Neurochemistry·Sylvia MontañezLynette C Daws
May 4, 2011·Journal of Neurochemistry·Nina NevalainenIngrid Strömberg
Mar 26, 2019·Journal of Alzheimer's Disease : JAD·Kevin N HascupErin R Hascup
Jan 26, 2010·Neurobiology of Disease·Sylvia NavaillesPhilippe De Deurwaerdère
Jun 26, 2008·Chemical Reviews·Donita L RobinsonR Mark Wightman
Jun 28, 2011·Experimental Neurology·J K MorrisJ A Stanford

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