In vivo electroporation of the testis versus transgenic mice model in functional studies of spermatocyte-specific hst70 gene promoter: A comparative study

Molecular Reproduction and Development
Wieslawa WidłakZdzislaw Krawczyk

Abstract

To determine whether DNA transfer to mouse testes by in vivo electroporation could be useful method for studying regulatory elements of genes specifically active in spermatocytes first we compared the expression pattern of a construct containing the EGFP reporter gene ligated to a fragment of the heat shock testis-specific hst70 gene promoter, both in testis of transgenic mice and in testis electroporated in vivo. While in transgenic mice the EGFP was expressed in all seminiferous tubules in a cell- and stage-specific manner, in the testes electroporated in vivo only small fraction of cells expressed this marker protein. In order to make a quantitative comparison between the specificity of these two experimental systems we used several vectors containing the CAT gene ligated to fragments of the hst70 gene 5' upstream of DNA sequences which either promoted or did not activate expression of the reporter gene in the testes of transgenic mice. Also, as a reference opposite to spermatogenic cells we examined the expression pattern of the same set of vectors in the rat hepatoma FTO 2B cells. Although electroporated testes retain some spermatocyte-specific features such as the ability to repress promoters which do not contain regulato...Continue Reading

References

Oct 8, 2011·DNA and Cell Biology·Nai-Zheng DingJianguo Chen
Mar 2, 2012·Biology of Reproduction·Xin WuAlexander Gow
Jul 16, 2008·Reproductive Biology and Endocrinology : RB&E·Emilio González-GonzálezJesús Del Mazo
Jul 1, 2009·Reproductive Biology and Endocrinology : RB&E·Sandra DannerRichard Ivell
Oct 4, 2006·International Journal of Andrology·Wieslawa WidlakZdzislaw Krawczyk
Oct 26, 2014·Cell Stress & Chaperones·Dorota Scieglinska, Zdzislaw Krawczyk
Sep 29, 2011·Molecular Reproduction and Development·Tushna DehnugaraM R Satyanarayana Rao

Citations

Jan 1, 1991·Mammalian Genome : Official Journal of the International Mammalian Genome Society·D J Wolgemuth, F Watrin
Jan 1, 1987·Molecular Biology Reports·Zdzislaw KrawczykE Biesiada
Oct 1, 1988·The Journal of Cell Biology·Zdzislaw KrawczykM Parvinen
Jan 1, 1982·Endocrine Reviews·M Parvinen
Apr 7, 1997·Biochemical and Biophysical Research Communications·T MuramatsuJ Okumura
Jan 5, 2000·The Journal of Experimental Zoology·Y YamazakiK Imoto
Jun 7, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·K SugiharaT Muramatsu

Related Concepts

Benign Neoplasm of Testis
Transfection
Heat-Shock Proteins 70
Neoplasm of Uncertain or Unknown Behavior of Testis
Liver Carcinoma
Promoter
House mice
Chloramphenicol Acetyl Transferase Gene
Gene Expression
Inferior Colliculus

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