In vivo estimation of cardiac transmembrane current

Circulation Research
F X WitkowskiR Plonsey

Abstract

The ionic currents that cross the myocardial membrane during cardiac activation have a corresponding return path in the extracellular space. The transmembrane current (Im) during activation of cardiac cells in situ has previously been envisioned only in mathematical models. We have developed a remarkably simple in vivo technique that incorporates an electrode array with cellular dimensions to continuously estimate the extracellular counterparts of cardiac Ims. Mathematical modeling was performed for uniform plane wave propagation to clarify the biophysical basis and underlying assumptions inherent in this approach. Five-element electrode arrays incorporating 75-microns-diameter silver electrodes with center-to-center distances of 210 microns were experimentally verified to provide spatially sufficient samples for voltage gradient determinations of myocardial activation. Similar results were obtained with 25-microns-diameter electrodes at a center-to-center spacing of 65 microns. An estimate of Im was obtained from the derivative of the magnitude of the voltage gradient of the measured interstitial potentials. The inward component of Im generated by normal Na+ channel activation at 37 degrees C was measured in vivo to be less th...Continue Reading

References

Mar 15, 1979·Nature·J J Colatsky, R W Tsien
Jan 1, 1978·Progress in Biophysics and Molecular Biology·G W Beeler, J A McGuigan
Apr 1, 1991·Journal of Electrocardiology·R Plonsey, A van Oosterom
Mar 1, 1990·Biophysical Journal·K T MurrayL M Hondeghem
Dec 1, 1985·Biophysical Journal·H WindischH A Tritthart
Jan 1, 1989·Journal of Electrocardiology·A van Oosterom, G J Huiskamp
Apr 1, 1987·The Journal of Physiology·A G Kléber, C B Riegger
Jan 1, 1966·Physiological Reviews·D Noble
Apr 1, 1970·Circulation Research·C R Vander Ark, E W Reynolds
Feb 1, 1984·Circulation Research·I R JosephsonA M Brown
Dec 1, 1984·IEEE Transactions on Bio-medical Engineering·R Plonsey
Jul 1, 1981·IEEE Transactions on Bio-medical Engineering·A E MarbleC W Hor
Sep 1, 1981·The Journal of Physiology·A M BrownT Powell
Apr 1, 1980·The Journal of General Physiology·L EbiharaE A Johnson
Dec 3, 1946·Proceedings of the Royal Society of Medicine·A L HODGKIN, W A H RUSHTON

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Citations

May 11, 2004·Journal of Electrocardiology·Mark PotseJacques M T de Bakker
Jan 1, 1995·Journal of Electrocardiology·H M PruenteS M Dillon
Jan 1, 1995·Pacing and Clinical Electrophysiology : PACE·C F Pieper, A Pacifico
May 1, 1995·Journal of Cardiovascular Electrophysiology·L J Leon, F X Witkowski
May 29, 2000·Journal of Cardiovascular Electrophysiology·S M Pogwizd
Nov 4, 2000·Journal of Cardiovascular Electrophysiology·R CoronelJ M de Bakker
Nov 4, 2000·Journal of Cardiovascular Electrophysiology·B B Punske
Mar 16, 2007·IEEE Transactions on Bio-medical Engineering·Barbara M Johnston, Peter R Johnston
Apr 9, 2008·IEEE Transactions on Bio-medical Engineering·Andrew E PollardWilliam M Smith
Jun 1, 1995·IEEE Transactions on Bio-medical Engineering·F X WitkowskiR Plonsey
Aug 1, 2000·IEEE Transactions on Bio-medical Engineering·J C Eason, R A Malkin
Oct 6, 1997·IEEE Engineering in Medicine and Biology Magazine : the Quarterly Magazine of the Engineering in Medicine & Biology Society·B He
Apr 25, 2000·Computers and Biomedical Research, an International Journal·M T YoungR E Ideker
Jan 22, 2013·Biophysical Journal·Richard A GrayJohn P Wikswo
Oct 8, 2013·Biophysical Journal·Richard A GrayJohn P Wikswo
Aug 7, 1995·Physical Review Letters·F X WitkowskiD T Kaplan
Jul 31, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Andrew E PollardRoger C Barr
Nov 14, 1998·The American Journal of Physiology·E MacchiB Taccardi
Aug 9, 2005·American Journal of Physiology. Heart and Circulatory Physiology·J James WileyAndrew E Pollard
Jul 19, 2000·American Journal of Physiology. Heart and Circulatory Physiology·R A Malkin, B D Pendley
Mar 10, 2001·The Annals of Thoracic Surgery·R P Scott

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