In vivo evaluation of the genotoxic effects of clomiphene citrate on rat reticulocytes: a micronucleus genotoxicity

Gynecologic and Obstetric Investigation
Bulent DuranAysel Guven

Abstract

To determine the genotoxic effects of clomiphene citrate (CC) on rat reticulocytesin vivo. In this prospective, randomized, controlled study, rats were each assigned randomly to the CC 50, CC 100, CC 200, or control group and were given repeat doses of 0.16, 0.32 or 0.64 mg CC, or normal saline, respectively. Each study group received its CC dose in 2 ml of saline intraperitoneally for 5 days, while the control group received only 2 ml of saline. Each treatment cycle was repeated six times. Six months later, the rats were euthanized. Bone marrow tissues were removed, and pluripotent reticulocyte cells with micronuclei, nuclear buds, and binuclear abnormalities were analyzed using an in situmicronuclei assay under light microscopy. The proportion of micronucleated erythrocytes was measured. Fewer cells with nuclear buds and binuclear abnormalities were detected in the CC 50 group and controls. The CC 100 and 200 groups had significantly (p < 0.05) more nuclear buds and binuclear abnormalities compared with the CC 50 group and controls in the cytogenetic analysis of bone marrow stem cells. In rats, the micronucleus genotoxicity assay suggests a dose-dependent CC effect on genomic instability in bone marrow stem cells in vivo.

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Citations

Nov 6, 2013·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Serkan YilmazSibel Erkal İlhan
Mar 7, 2014·Cytotechnology·Serkan YilmazEbru Yilmaz
Dec 3, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·S ÖzdenM Özel
Apr 1, 2011·Current Therapeutic Research, Clinical and Experimental·Bulent DuranOzturk Ozdemir
Nov 22, 2017·Cytotechnology·Serkan YilmazEbru Yilmaz
May 11, 2018·Drug and Chemical Toxicology·Eyyüp Rencüzoğulları, Muhsin Aydın
Jun 1, 2021·Chemico-biological Interactions·Sharmistha ChoudhuriShailendra Asthana

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