PMID: 9436609Jan 22, 1998Paper

In vivo evidence of free radical formation after asbestos instillation: an ESR spin trapping investigation

Free Radical Biology & Medicine
A J GhioR P Mason

Abstract

It has been postulated that the in vivo toxicity of asbestos results from its catalysis of free radical generation. We examined in vivo radical production using electron spin resonance (ESR) coupled with the spin trap alpha-(4-pyridyl-1-oxide)-N-t-butylnitrone (4-POBN); 180 day-old rats were intratracheally instilled with either 500 microg crocidolite or saline. Twenty-four hours later, histologic examination revealed a neutrophilic inflammatory response. ESR spectroscopy of the chloroform extract from lungs exposed to asbestos gave a spectrum consistent with a carbon-centered radical adduct, while those spectra from lungs instilled with saline revealed a much weaker signal. This same radical formation persisted and, even one month after instillation, could be detected in the lungs of rats exposed to asbestos. The 4-POBN adducts detected by ESR are very similar to, if not identical with, ethyl and pentyl radical adducts, providing evidence of in vivo lipid peroxidation resulting from asbestos exposure. We conclude that, after instillation of crocidolite in the rat, ESR analysis of lung tissue demonstrates in vivo free radical production.

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Citations

Apr 23, 2003·Free Radical Biology & Medicine·Arti ShuklaBrooke T Mossman
Mar 3, 2005·Journal of Toxicology and Environmental Health. Part a·Francesco TurciBice Fubini
Nov 15, 2007·Antioxidants & Redox Signaling·Fei GaoTim D Oury
Jun 19, 2008·American Journal of Physiology. Endocrinology and Metabolism·Krisztian StadlerMaria B Kadiiska
Oct 13, 2004·American Journal of Respiratory and Critical Care Medicine·Toyoko ArimotoRonald P Mason
Aug 31, 2011·Critical Reviews in Clinical Laboratory Sciences·Ivan Spasojević
Jul 26, 2005·Inhalation Toxicology·David BernsteinUNKNOWN ILSI Risk Science Institute Working Group
Jun 24, 2009·Critical Reviews in Toxicology·Ken Donaldson
May 12, 2004·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·M B KadiiskaR P Mason
Apr 7, 2000·Environmental Health Perspectives·B RihnG Keith
Nov 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Keizo SatoRonald P Mason

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