In vivo evidence of free radical generation in the mouse lung after exposure to Pseudomonas aeruginosa bacterium: an ESR spin-trapping investigation.

Free Radical Research
Keizo SatoM B Kadiiska

Abstract

In the Pseudomonas aeruginosa-induced rodent pneumonia model, it is thought that free radicals are significantly associated with the disease pathogenesis. However, until now there has been no direct evidence of free radical generation in vivo. Here we used electron spin resonance (ESR) and in vivo spin trapping with α-(4-pyridyl-1-oxide)-N-tert-butylnitrone to investigate free radical production in a murine model. We detected and identified generation of lipid-derived free radicals in vivo (a(N) =14.86 ± 0.03 G and a(H)(β) =2.48 ± 0.09 G). To further investigate the mechanism of lipid radical production, we used modulating agents and knockout mice. We found that with GdCl(3) (phagocytic toxicant), NADPH-oxidase knockout mice (Nox2(-)/(-)), allopurinol (xanthine-oxidase inhibitor) and Desferal (metal chelator), generation of lipid radicals was decreased; histopathological and biological markers of acute lung injury were noticeably improved. Our study demonstrates that lipid-derived free radical formation is mediated by NADPH-oxidase and xanthine-oxidase activation and that metal-catalysed hydroxyl radical-like species play important roles in lung injury caused by Pseudomonas aeruginosa.

References

Aug 2, 1990·Nature·T A Springer
Dec 1, 1989·Biochemical Society Transactions·A HartleyC Rice-Evans
Apr 25, 1989·Biochimica Et Biophysica Acta·M Wajner, R A Harkness
Sep 1, 1987·Basic Research in Cardiology·M Muxfeldt, W Schaper
Aug 1, 1995·American Journal of Respiratory Cell and Molecular Biology·K J PendinoD L Laskin
Jun 1, 1994·Journal of Magnetic Resonance. Series B·D R Duling
Jun 1, 1993·Archives of Biochemistry and Biophysics·K T Knecht, R P Mason
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·T AkaikeH Maeda
May 1, 1997·European Journal of Biochemistry·S A SandersR Harrison
Jul 9, 1998·American Journal of Respiratory and Critical Care Medicine·P M HassounJ J Lanzillo
Feb 3, 1999·Lung·R K Bouhafs, C Jarstrand
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·A E DikalovaR P Mason
Jan 10, 2002·Microbial Pathogenesis·Zacharias E SuntresPang N Shek
Nov 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Keizo SatoRonald P Mason
Sep 12, 2003·Trends in Pharmacological Sciences·Hua CaiDavid G Harrison
Sep 27, 2003·American Journal of Respiratory Cell and Molecular Biology·Richard M WrightJohn E Repine
Nov 11, 2003·Free Radical Biology & Medicine·Rheal A TownerRonald P Mason
Mar 9, 2006·Photochemistry and Photobiology·Kozo NakaiColin F Chignell
Jan 1, 2008·Free Radical Biology & Medicine·Albert van der Vliet
Nov 26, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Houssam RaadJamel El-Benna
Dec 18, 2008·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Nobuyuki ShimonoJun Hayashi
May 14, 2009·Vascular Health and Risk Management·Jacob George, Allan D Struthers
Aug 21, 2010·Respirology : Official Journal of the Asian Pacific Society of Respirology·Bryan J WilliamsTimothy S Blackwell
Sep 15, 2010·Cell and Tissue Research·Andreas SchmiedlThomas Tschernig
Oct 19, 2010·International Journal of Medical Microbiology : IJMM·Alessandra Bragonzi

❮ Previous
Next ❯

Citations

Jul 25, 2013·Geriatrics & Gerontology International·Toru SasakiShuji Kojima
Jun 12, 2020·Journal of Clinical Biochemistry and Nutrition·Satomi AkazakiKeizo Sato

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.