PMID: 8583152Nov 1, 1995Paper

In vivo evidence that intercellular adhesion molecule-1 does not mediate endotoxin-induced hepatic leukocyte-endothelial cell interaction

Journal of Hepatology
B VollmarM D Menger

Abstract

We have previously demonstrated that endotoxemia induces an immediate leukocytic response within the hepatic microvasculature, with leukostasis in sinusoids and leukocyte adherence in post-sinusoidal venules. We have now studied the role of intercellular adhesion molecule-1 in endotoxin-induced hepatic leukocyte-endothelial cell interaction in vivo using fluorescence microscopy. Sprague-Dawley rats were pretreated with either 1 mg/kg (n = 5) or 2 mg/kg (n = 4) of a monoclonal anti-rat anti-intercellular adhesion molecule-1 antibody intravenously, followed by exposure to endotoxin (E. coli LPS 10 mg/kg iv). Animals pretreated with an isotype-matched IgG1 control antibody (n = 5) served as controls. Intravital fluorescence microscopy for analysis of the hepatic microcirculation was performed prior to and 1 h after lipopolysaccharide exposure. At 1 h after lipopolysaccharide-exposure, control animals showed a marked reduction of systemic leukocyte count, which was associated with a significant (p < 0.01) hepatic microvascular accumulation of leukocytes with stasis in sinusoids, as well as rolling and adherence in postsinusoidal venules. Pretreatment with anti-intercellular adhesion molecule-1 was not effective in preventing either...Continue Reading

References

Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M P BevilacquaM A Gimbrone
Jul 1, 1994·The Journal of Experimental Medicine·H XuJ C Gutierrez-Ramos

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Citations

Aug 26, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J J JonesO D Rotstein
Nov 5, 2010·Cell and Tissue Research·Paulina M KowalewskaAlison E Fox-Robichaud
May 26, 1999·Infectious Disease Clinics of North America·C Parent, P Q Eichacker
Jun 4, 2013·The Journal of Surgical Research·Edward Castro-SantaEduard Ryschich
Dec 12, 2012·Microvascular Research·Franziska Timm, Brigitte Vollmar
May 1, 1996·The British Journal of Surgery·M D Menger, B Vollmar
Jan 25, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shinsuke MiyaharaAli Hafezi-Moghadam
Dec 4, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lama AlmulkiAli Hafezi-Moghadam
Apr 1, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·T SchäferB Vollmar
May 12, 1998·The American Journal of Physiology·J G ChosayH Jaeschke

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