PMID: 9181463May 1, 1997Paper

In vivo expression of an interferon-alpha gene by intramuscular injection of naked DNA

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
C M LawsonM W Beilharz

Abstract

Acid-stable type I interferons belong to a multigene family. The biologic relevance of each subtype in vivo remains unknown. We have developed an experimental model in which muscles were transfected in situ with naked DNA plasmids encoding an IFN transgene to assess the roles of individual IFN subtypes in vivo. Murine IFN-alpha 9 gene was subcloned into several mammalian expression vectors. Adult C57BL/6 mice were injected bilaterally in regenerating tibialis anterior muscles with naked DNA 5 days after muscle injury to enhance DNA uptake and expression. In the muscles of mice given the IFN-alpha 9 plasmid constructs, acid-stable IFNs were detected by bioassay using reduction in cytopathic effect of encephalomyocarditis virus-infected L929 cells. In these same muscles, IFN-alpha 9 transcripts were identified by RT-PCR, indicating that transcription had occurred. Acid-stable IFNs were detected from days 7 to 28 post-DNA inoculation. Furthermore, these proteins were found in the sera of DNA-inoculated mice. Control groups of mice given the blank expression vectors did not produce detectable IFNs in muscle or sera as determined by bioassay, nor were transcripts detected by RT-PCR. This approach now allows investigation of the effe...Continue Reading

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Citations

Sep 3, 1998·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·J W LowenthalJ J York
May 8, 2003·Biological Procedures Online·Emmalene J. BartlettCassandra M. James
Sep 29, 2001·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·E BosioM W Beilharz
Aug 10, 1997·Human Gene Therapy·L M BlechyndenC M Lawson
Jul 9, 2002·Immunology·Vanessa S CullCassandra M James
Jun 8, 2001·Experimental Biology and Medicine·D J CarrB M Gebhardt
Sep 7, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·C M Lawson, M W Beilharz
Sep 13, 2002·Immunology and Cell Biology·Emmalene J BartlettCassandra M James
Jan 24, 2006·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Peter J BuontempoBahige M Baroudy
May 4, 2001·Journal of Autoimmunity·D FairweatherN R Rose

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