In vivo expression of proteases and protease inhibitor, a serpin, by periodontal pathogens at teeth and implants

Molecular Oral Microbiology
M EckertSigrun Eick

Abstract

Porphyromonas gingivalis and Tannerella forsythia secrete proteases, gingipains and KLIKK-proteases. In addition, T. forsythia produces a serpin (miropin) with broad inhibitory spectrum. The aim of this pilot study was to determine the level of expression of miropin and individual proteases in vivo in periodontal and peri-implant health and disease conditions. Biofilm and gingival crevicular fluid (GCF)/ peri-implant sulcular fluid (PISF) samples were taken from healthy tooth and implant sites (n = 10), gingivitis and mucositis sites (n = 12), and periodontitis and peri-implantitis sites (n = 10). Concentration of interleukin-8 (IL-8), IL-1β and IL-10 in GCF was determined by enzyme-linked immunosorbent assay. Loads of P. gingivalis and T. forsythia and the presence of proteases and miropin genes were assessed in biofilm by quantitative PCR, whereas gene expression was estimated by quantitative RT-PCR. The presence of P. gingivalis and T. forsythia, as well as the level of IL-8 and IL-1β, were associated with disease severity in the periodontal and peri-implant tissues. In biofilm samples harboring T. forsythia, genes encoding proteases were found to be present at 72.4% for karilysin and 100% for other KLIKK-protease genes and ...Continue Reading

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Citations

Feb 15, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Sigrun EickAndreas Stavropoulos
Jan 10, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alicja Sochaj-GregorczykJan Potempa
Jul 3, 2021·International Journal of Molecular Sciences·Héla MkaouarMoez Rhimi

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