In vivo function of a gammaherpesvirus virion glycoprotein: influence on B-cell infection and mononucleosis

Journal of Virology
James P StewartH Adler

Abstract

The human gammaherpesviruses Epstein-Barr virus and Kaposi Sarcoma-associated herpesvirus both contain a glycoprotein (gp350/220 and K8.1, respectively) that mediates binding to target cells and has been studied in great detail in vitro. However, there is no direct information on the role that these glycoproteins play in pathogenesis in vivo. Infection of mice by murid herpesvirus 4 strain 68 (MHV-68) is an established animal model for gammaherpesvirus pathogenesis and expresses an analogous glycoprotein, gp150. To elucidate the in vivo function of gp150, a recombinant MHV-68 deficient in gp150 production was generated (vgp150Delta). The productive viral replication in vitro and in vivo was largely unaffected by mutation of gp150, aside from a partial defect in the release of extracellular virus. Likewise, B-cell latency was established. However, the transient mononucleosis and spike in latently infected cells associated with the spread of MHV-68 to the spleen was significantly reduced in vgp150Delta-infected mice. A soluble, recombinant gp150 was found to bind specifically to B cells but not to epithelial cells in culture. In addition, gp150-deficient MHV-68 derived from mouse lungs bound less well to spleen cells than wild-ty...Continue Reading

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Citations

Jul 12, 2012·Archives of Virology·David J HughesJames P Stewart
Aug 19, 2010·Immunologic Research·Ting-Ting WuRen Sun
Mar 21, 2008·Journal of Virology·Inga DryGeorge C Russell
Sep 28, 2007·Journal of Virology·Laurent Gillet, Philip G Stevenson
Mar 30, 2005·Journal of Virology·Janet S MayPhilip G Stevenson
Dec 8, 2005·Veterinary Microbiology·Mathias Ackermann
May 5, 2007·Biotechnology Letters·Teruaki KobayashiTatsurou Shibui
Dec 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Basak KayhanMarcia A Blackman
Nov 23, 2019·Viruses·Stephen J Dollery
Dec 16, 2006·The Journal of General Virology·Jane HartJames P Stewart

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