PMID: 9539720May 16, 1998Paper

In vivo function of mutated spliced leader RNAs in Caenorhabditis elegans

Proceedings of the National Academy of Sciences of the United States of America
H Xie, D Hirsh

Abstract

The role of spliced leader RNA (SL RNA) in trans-splicing in Caenorhabditis elegans has been studied through a combination of in vitro mutagenesis and in vivo complementation of rrs-1 mutant nematodes, which lack endogenous SL1 RNA. Three classes of mutant SL1 RNAs have been found-those that rescue the lethal phenotype at low concentration of transforming DNA, those that rescue at high but not low concentration, and those that do not rescue at all. These studies showed that some mutations in the otherwise highly conserved 22-nt spliced leader are tolerated for splicing and post-splicing events. A longer spliced leader also can be tolerated but only when present in high copy number. Changes in the first 16 nucleotides result in the appearance of no SL RNA, consistent with the in vitro studies by others showing that the SL1 RNA promoter partly resides within the spliced leader sequence.

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Citations

Sep 12, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Eva L ArkbladJan Rydström
Aug 25, 2000·Molecular and Cellular Biology·D Evans, T Blumenthal
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·N A Stover, R E Steele
Oct 1, 2011·Wiley Interdisciplinary Reviews. RNA·Erika L Lasda, Thomas Blumenthal
Sep 5, 2006·International Journal for Parasitology·Alena Mayo IñiguezAna Carolina Paulo Vicente
Jun 26, 1999·The Journal of Biological Chemistry·N R Sturm, D A Campbell

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