In vivo functional analysis of the Drosophila melanogaster nicotinic acetylcholine receptor Dα6 using the insecticide spinosad

Insect Biochemistry and Molecular Biology
Jason SomersTrent Perry

Abstract

The vinegar fly, Drosophila melanogaster, has been used to identify and manipulate insecticide resistance genes. The advancement of genome engineering technology and the increasing availability of pest genome sequences has increased the predictive and diagnostic capacity of the Drosophila model. The Drosophila model can be extended to investigate the basic biology of the interaction between insecticides and the proteins they target. Recently we have developed an in vivo system that permits the expression and study of key insecticide targets, the nicotinic acetylcholine receptors (nAChRs), in controlled genetic backgrounds. Here this system is used to study the interaction between the insecticide spinosad and a nAChR subunit, Dα6. Reciprocal chimeric subunits were created from Dα6 and Dα7, a subunit that does not respond to spinosad. Using the in vivo system, the Dα6/Dα7 chimeric subunits were tested for their capacity to respond to spinosad. Only the subunits containing the C-terminal region of Dα6 were able to respond to spinosad, thus confirming the importance this region for spinosad binding. A new incompletely dominant, spinosad resistance mechanism that may evolve in pest species is also examined. First generated using che...Continue Reading

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Citations

Nov 3, 2016·Journal of Neurogenetics·Richard H Ffrench-ConstantChris Bass
Jan 22, 2017·Journal of Insect Physiology·Clauvis Nji Tizi TaningGuy Smagghe
Oct 9, 2015·Wiley Interdisciplinary Reviews. Developmental Biology·Koen J T VenkenKristi L Hoffman
Aug 20, 2017·Journal of Pesticide Science·Andrew J CrossthwaitePeter Maienfisch
May 21, 2020·Pest Management Science·Ana Guillem-AmatFélix Ortego
Jun 23, 2020·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Guilu Zhang, Wenjun Zhang
Dec 9, 2016·Proceedings of the National Academy of Sciences of the United States of America·Vassilis DourisJohn Vontas
Nov 18, 2020·Pest Management Science·Jianqiang LanQian Han
Jan 18, 2018·Insect Biochemistry and Molecular Biology·Xi'en ChenYongping Huang
Jul 2, 2021·Ecotoxicology and Environmental Safety·Shuangyan YaoShiheng An

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