PMID: 15223795Jun 30, 2004Paper

In vivo gene delivery and visualization of corneal stromal cells using an adenoviral vector and keratocyte-specific promoter

Investigative Ophthalmology & Visual Science
Eric C CarlsonVictor L Perez

Abstract

This study was conducted to determine whether intrastromal injection of adenoviral construct could be used to transfect corneal stroma cells effectively in vivo and to determine whether a tissue-specific promoter could be used to express exogenous genes in keratocytes. An adenoviral construct with a cytomegalovirus (pCMV)-driven enhanced green fluorescent protein (EGFP) reporter gene was injected into the stroma of murine corneas. In vivo expression was quantitated and samples were analyzed by in vivo stereomicroscopy, and ex vivo expression was determined by confocal three dimensional (3-D) reconstruction. The 3.2-kb keratocan promoter was used to drive tissue-specific reporter gene expression in vivo. EGFP expression was first detected in vivo 11 hours after injection of adeno-EGFP in the corneal stroma, with a duration of approximately 3 weeks. Ex vivo wholemount cornea confocal analysis with 3-D reconstruction allowed visualization of EGFP expression in corneal stroma cells, to accurately assess cellular architecture and distribution in the corneal stroma. Naked pCMV-EGFP plasmid DNA did not express the reporter gene to the levels of the adeno-EGFP. The 3.2-kb keratocan promoter was capable of driving EGFP tissue-specific e...Continue Reading

Citations

Jun 16, 2005·Progress in Retinal and Eye Research·Rajiv R MohanSteven E Wilson
Jun 22, 2012·European Journal of Medical Research·Mario MatthaeiAlbert S Jun
Mar 13, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Nicolas SerratriceEric J Kremer
Jul 11, 2013·The Ocular Surface·Rajiv R MohanAjay Sharma
Jun 30, 2009·Brain Research Bulletin·Jinsong HaoChia-Yang Liu
Oct 27, 2015·Experimental Eye Research·Xianling TangPing Liu
Aug 21, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Eytan A KlausnerShridhar V Andurkar
Oct 22, 2013·Seminars in Ophthalmology·Yureeda Qazi, Pedram Hamrah
Oct 5, 2011·Progress in Retinal and Eye Research·Rajiv R MohanAshish Tandon
Aug 29, 2007·The Journal of Gene Medicine·Yaw-Chong TongJiahorng Liaw
Jul 14, 2006·Gene Therapy·J W B BainbridgeR R Ali
Oct 11, 2017·Cornea·Victor L Perez
Apr 15, 2020·Pharmaceutics·Fernando Alvarez-RiveraCarmen Alvarez-Lorenzo
Apr 26, 2005·The Journal of Biological Chemistry·Eric C CarlsonWinston W-Y Kao
Apr 16, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·M WelsF Sauvage

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