In vivo gene transfer by electroporation allows expression of a fluorescent transgene in hamster testis and epididymal sperm and has no adverse effects upon testicular integrity or sperm quality

Biology of Reproduction
Olivia HibbittJohn Parrington

Abstract

The study of gene function in testis and sperm has been greatly assisted by transgenic mouse models. Recently, an alternative way of expressing transgenes in mouse testis has been developed that uses electroporation to introduce transgenes into the male germ cells. This approach has been successfully used to transiently express reporter genes driven by constitutive and testis-specific promoters. It has been proposed as an alternative method for studying gene function in testis and sperm, and as a novel way to create transgenic animals. However, the low levels and transient nature of transgene expression that can be achieved using this technique have raised concerns about its practical usefulness. It has also not been demonstrated in mammals other than mice. In this study, we show for the first time that in vivo gene transfer using electroporation can be used to express a fluorescent transgene in the testis of a mammal other than mice, the Syrian golden hamster. Significantly, for the first time we demonstrate expression of a transgene in epididymal sperm using this approach. We show that expression of the transgene can be detected in sperm for as long as 60 days following gene transfer. Finally, we provide the first systematic ...Continue Reading

References

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Citations

Apr 15, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jochen De VryJos Prickaerts
Jul 1, 2009·Reproductive Biology and Endocrinology : RB&E·Sandra DannerRichard Ivell
Dec 21, 2007·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Sep 14, 2007·Archives of Andrology·Kevin CowardJohn Parrington
Jan 13, 2011·Systems Biology in Reproductive Medicine·John ParringtonJoaquin Gadea

Related Concepts

Epididymis
Cricetus
Immunofluorescence Microscopy
Sperm Motility
Spermiogenesis
Y-Chromosome-Bearing Sperm
Testis
Gene Transfer Techniques
Electroporation
Recombinant Transgenes

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