In vivo gene transfer to mouse spermatogenic cells using green fluorescent protein as a marker

The Journal of Experimental Zoology
Y YamazakiK Imoto


Combination of the DNA injection into seminiferous tubules and the subsequent in vivo electroporation (EP) has become an efficient and convenient assay system for spermatogenic-specific gene expression during spermatogenesis of mice. In this study, we made methodological modifications to enhance the transfection efficiency, and evaluated the possibility of this technique to generate transgenic offspring using green fluorescent protein (GFP) as a marker. After the in vivo gene transfer, GFP expression could be monitored easily and repeatedly on the surface of the testis of live mice under fluorescent microscopy. The serial sections of the transfected testis revealed that transient expression of GFP was extended even in the innermost region of the testis uniformly, but confined to spermatogenic cells and Sertoli cells within the seminiferous tubules. Furthermore, long-lasting GFP expression could be detected in the spermatogenic cells even 2 months after EP. Natural mating with normal adult females revealed that 65% of the transfected males maintained fertilizable ability and could generate their offspring normally. Germ-line transmission of the GFP vector to the offspring was checked under fluorescent microscopy, but no transgen...Continue Reading


Jan 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kayoko KuritaNoriyoshi Sakai
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·M YangR M Hoffman
Jul 16, 2008·Reproductive Biology and Endocrinology : RB&E·Emilio González-GonzálezJesús Del Mazo
Jul 1, 2009·Reproductive Biology and Endocrinology : RB&E·Sandra DannerRichard Ivell
Oct 18, 2011·Asian Journal of Andrology·Zhen ZhangFei Zhou
Jul 12, 2002·The Journal of Gene Medicine·Markus DüchlerMathias Müller
Mar 21, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Terje SvingenPeter Koopman
May 17, 2008·Development, Growth & Differentiation·Kentaro Yomgogida
Jul 29, 2004·Molecular and Biochemical Parasitology·Jason M Correnti, Edward J Pearce
Oct 11, 2005·Experimental Parasitology·Xiao-Song YuanMing-Seng Jiang
Jun 14, 2005·Developmental Biology·Masanobu ShojiN Nakatsuji
Sep 14, 2007·Archives of Andrology·Kevin CowardJohn Parrington
Jun 16, 2000·FEBS Letters·M NaganoR L Brinster
Aug 24, 2002·Biology of Reproduction·Kentaro YomogidaYoshitake Nishimune
Jun 8, 2006·Physics in Medicine and Biology·P T Gaynor, P S Bodger
Jan 16, 2008·Molecular Reproduction and Development·Arunima MaitiG C Majumder
Mar 18, 2003·Genesis : the Journal of Genetics and Development·Masahiro SatoMinoru Kimura
Feb 17, 2005·Journal of Andrology·Yukihiro UmemotoKenjiro Kohri
Jan 13, 2011·Systems Biology in Reproductive Medicine·John ParringtonJoaquin Gadea


Jan 1, 1991·Annals of the New York Academy of Sciences·D J WolgemuthF Watrin
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·A R ThierryL C Mahan
Aug 25, 1995·Science·E Marshall
May 1, 1993·Trends in Biotechnology·V J DzauG H Gibbons
Apr 1, 1995·Biology of Reproduction·Y Kimura, R Yanagimachi
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·R HeimR Y Tsien
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·R L Brinster, M R Avarbock
Oct 1, 1995·Biology of Reproduction·Y Kimura, R Yanagimachi
Jul 8, 1996·FEBS Letters·R HellerC Nicolau
Apr 1, 1997·Molecular Reproduction and Development·J H KimK S Chung
Feb 1, 1997·Biology of Reproduction·K T Blanchard, K Boekelheide
Apr 7, 1997·Biochemical and Biophysical Research Communications·T MuramatsuJ Okumura
Feb 24, 1998·Biology of Reproduction·I SasagawaR Yanagimachi
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·A OguraJ Matsuda

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Gene Transfer Techniques
Green Fluorescent Proteins
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