In vivo gene transfer to mouse spermatogenic cells by deoxyribonucleic acid injection into seminiferous tubules and subsequent electroporation

Biology of Reproduction
Y YamazakiT Noce

Abstract

An in vivo gene transfer technique for living mouse testes was used to develop a novel transient expression assay system for transcriptional regulatory elements of spermatogenic specific genes. The combination of DNA injection into seminiferous tubules and subsequent in vivo electroporation resulted in an efficient and convenient assay system for gene expression during spermatogenesis. The transfer of the firefly luciferase reporting gene driven by the Protamine-1 (Prm-1) enhancer region revealed a significant increase in the activity of the reporter enzyme. Histochemical studies of the transfer of the lacZ gene driven by the Prm-1 enhancer showed specific lacZ expression only in haploid spermatid cells in adult testes, corresponding with the expression pattern of endogenous Prm-1. We were able to detect long-lasting transgene expression in the transfected spermatogenic cells. A group of spermatogenic differentiating cells maintained the transfected lacZ expression after more than 2 mo of transfection, suggesting that spermatogenic stem cells and/or spermatogonia could also incorporate foreign DNA and that the transgene could be transmitted to the progenitor cells derived from a transfected proliferating germ cell.

References

Oct 29, 2011·Biochemical and Biophysical Research Communications·Xiang-Yang Miao, Xin Zhang
Jun 7, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·K SugiharaT Muramatsu
Oct 18, 2011·Asian Journal of Andrology·Zhen ZhangFei Zhou
Feb 8, 2003·DNA and Cell Biology·Ann E O Trezise
Aug 24, 2002·Biology of Reproduction·Kentaro YomogidaYoshitake Nishimune
Feb 27, 2003·Biology of Reproduction·M P De Miguel, P J Donovan
Feb 28, 2003·Biology of Reproduction·Catherine CelebiBernard Jégou
Apr 9, 2004·Biology of Reproduction·Jon M OatleyDerek J McLean
Jun 11, 2004·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
May 17, 2008·Development, Growth & Differentiation·Kentaro Yomgogida
Jul 16, 2008·Reproductive Biology and Endocrinology : RB&E·Emilio González-GonzálezJesús Del Mazo
Jul 1, 2009·Reproductive Biology and Endocrinology : RB&E·Sandra DannerRichard Ivell
Jan 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kayoko KuritaNoriyoshi Sakai
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·M NaganoR L Brinster
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mito Kanatsu-ShinoharaTakashi Shinohara
Oct 7, 2006·Ontogenez·I P SavchenkovaL K Ernst
Feb 11, 2012·Systems Biology in Reproductive Medicine·Masahiro SatoSatoshi Watanabe
Sep 14, 2007·Archives of Andrology·Kevin CowardJohn Parrington
Jan 13, 2011·Systems Biology in Reproductive Medicine·John ParringtonJoaquin Gadea
Jul 12, 2002·Molecular Reproduction and Development·Catherine CelebiThierry Guillaudeux
Jul 12, 2002·The Journal of Gene Medicine·Markus DüchlerMathias Müller
Aug 5, 2008·Science in China. Series C, Life Sciences·Bichun LiGuohong Chen
Dec 24, 2008·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Yidong Niu, Shulong Liang
Sep 17, 2005·Theriogenology·Sun-A OckGyu-Jin Rho
Jun 14, 2005·Developmental Biology·Masanobu ShojiN Nakatsuji
Sep 24, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Song ChenDongchun Liang
Dec 12, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·V R ArrudaK A High
Jan 20, 2017·Animal Biotechnology·Samantha N LottiMatthew B Wheeler
Jan 5, 2000·The Journal of Experimental Zoology·Y YamazakiK Imoto
Jun 16, 2000·FEBS Letters·M NaganoR L Brinster
Jan 12, 2001·Neurologia Medico-chirurgica·T KondohN Tamaki

Citations

Jan 1, 1991·Annals of the New York Academy of Sciences·D J WolgemuthF Watrin
Jan 1, 1991·Annals of the New York Academy of Sciences·M K SkinnerC T Anthony
Jan 1, 1991·Mammalian Genome : Official Journal of the International Mammalian Genome Society·D J Wolgemuth, F Watrin
Oct 1, 1989·Biology of Reproduction·K H ThomasM I Simon
Oct 1, 1989·Biology of Reproduction·L D Russell, A Steinberger
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M O RobinsonM I Simon
Jan 1, 1988·Journal of Cell Science. Supplement·D G De Rooij
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·A R ThierryL C Mahan
Aug 25, 1995·Science·E Marshall
May 1, 1993·Trends in Biotechnology·V J DzauG H Gibbons
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·R L Brinster, M R Avarbock
Jun 1, 1993·Current Opinion in Genetics & Development·K F Kozarsky, J M Wilson
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B P ZambrowiczR D Palmiter
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·K LeeR E Braun
Aug 1, 1996·Molecular and Cellular Biology·A Dupressoir, T Heidmann
Jul 8, 1996·FEBS Letters·R HellerC Nicolau
Feb 1, 1997·Biology of Reproduction·K T Blanchard, K Boekelheide
Apr 7, 1997·Biochemical and Biophysical Research Communications·T MuramatsuJ Okumura

Related Concepts

Study
In Vivo
Microinjections
Transcriptional Regulation
Transfection
Gene Transfer Techniques
Protamine Sulfate (USP)
Genes
Injection Procedure
Gene Expression

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.