In vivo generation of DNA sequence diversity for cellular barcoding

BioRxiv : the Preprint Server for Biology
Ian D PeikonAnthony M Zador

Abstract

Heterogeneity is a ubiquitous feature of biological systems. A complete understanding of such systems requires a method for uniquely identifying and tracking individual components and their interactions with each other. We have developed a novel method of uniquely tagging individual cells in vivo with a genetic “barcode” that can be recovered by DNA sequencing. We demonstrate the feasibility of this technique in bacterial cells. This method should prove useful in tracking interactions of cells within a network, and/or heterogeneity within complex biological samples.

Related Concepts

Molecular Probe Techniques
DNA Sequence
Tracking
Cellularization
Human DNA Sequencing
In Vivo
DNA Barcoding, Taxonomic

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