In vivo heme scavenging by Staphylococcus aureus IsdC and IsdE proteins

Biochemical and Biophysical Research Communications
John MackMartin J Stillman

Abstract

We report the first characterization of the in vivo porphyrin scavenging abilities of two components of a newly discovered heme scavenging system involving iron-regulated surface determinant (Isd) proteins. These proteins are present within the cell envelope of the Gram-positive human pathogen Staphylococcus aureus. IsdC and IsdE, when expressed heterologously in Escherichia coli, efficiently scavenged intracellular heme and resulted in de novo heme synthesis in excess of 100-fold above background. Magnetic circular dichroism analyses showed that the heme-binding properties of the two proteins differ significantly from one another. IsdC bound almost exclusively free-base protoporphyrin IX, whereas the IsdE protein was associated with low spin Fe(III) and Fe(II) heme. These properties provide important insight into the possible mechanisms of iron scavenging from bound heme by Isd proteins.

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Citations

Dec 2, 2011·Seminars in Immunopathology·Andrea DeDentOlaf Schneewind
Sep 19, 2012·Journal of the American Chemical Society·Michael T TiedemannMartin J Stillman
May 31, 2007·Natural Product Reports·Angela Wilks, Kimberly A Burkhard
Jul 5, 2011·Metallomics : Integrated Biometal Science·Christopher L Nobles, Anthony W Maresso
Nov 3, 2004·The Journal of Biological Chemistry·Ruiying WuAndrzej Joachimiak
Aug 21, 2008·The Journal of Biological Chemistry·Woo Cheol LeeMichael E P Murphy
Aug 22, 2008·The Journal of Biological Chemistry·Valerie A VillarealRobert T Clubb
Aug 5, 2008·The Journal of Biological Chemistry·Naomi MuryoiMartin J Stillman
Mar 10, 2006·Microbiology and Molecular Biology Reviews : MMBR·Luciano A MarraffiniOlaf Schneewind
Mar 14, 2012·PLoS Pathogens·MarCia T EkworomaduAnthony W Maresso
Aug 26, 2014·PloS One·Erin S HonsaSarah K Highlander
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Jan 16, 2008·Journal of Inorganic Biochemistry·Mark PluymMartin J Stillman

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