Abstract
The aim of this study was to analyze the hepatic differentiation potential of human umbilical cord blood-derived mesenchymal stem cells (hUCBMSCs) after transplantation into severe combined immune deficiency (SCID) mice with liver injury induced by D-galactosamine/lipopolysaccharide (GalN/LPS) and to explore the possibility that cells can partially repair GalN/LPS-induced hepatic damage. Mononuclear cells (MNCs) were isolated from fresh human umbilical cord blood, characterized by flow cytometry, and then transplanted into GalN/LPS-injured mice. Specimens were collected at 7, 14, 21, and 28 days after hUCBMSC transplantation. Histopathological changes were analyzed by hematoxylin and eosin staining. Polymerase chain reaction (PCR) for a specific marker of human cells, the human Alu sequence, was performed to locate exogenous hUCBMSCs in mouse livers. Expression of human hepatocyte-specific markers such as human albumin (hALB), human alpha-fetoprotein (hAFP), human cytokeratin 18 (hCK18), and human cytokeratin 19 (hCK19) were analyzed by reverse transcriptase (RT)-PCR and immunohistochemical staining. The hUCBMSCs were positive for the human MSC-specific markers CD271, CD29, CD90, CD105, and CD73, but negative for CD31, CD79b, C...Continue Reading
References
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C GalanosW Reutter
Jul 27, 1999·Life Sciences·Q XiongS Kadota
Nov 11, 1999·Journal of Biomedical Materials Research·H Ohgushi, A I Caplan
Feb 24, 2001·The New England Journal of Medicine·R QuartoM Marcacci
Apr 17, 2001·Tissue Engineering·P A ZukM H Hedrick
Apr 27, 2001·Mechanisms of Ageing and Development·M S Rao, M P Mattson
Aug 6, 2002·Experimental Hematology·Yuehua JiangCatherine M Verfaillie
Nov 29, 2002·Stem Cells·Suzanne KadereitMary J Laughlin
Feb 20, 2003·Leukemia·L FouillardN-C Gorin
Oct 25, 2003·Blood·Oscar K LeeTain-Hsiung Chen
Jul 28, 2004·Stem Cells·Karen BiebackHermann Eichler
Sep 11, 2004·Biochemical and Biophysical Research Communications·Eun Ji GangHoeon Kim
Nov 16, 2006·Stem Cells and Development·Yun-Jae JungHo-Seong Han
Aug 22, 2007·The British Journal of Radiology·M MouiseddineA Chapel
Nov 9, 2007·Gene Therapy·B M Abdallah, M Kassem
Feb 5, 2008·Gastroenterology·David CampardEtienne Marc Sokal
Dec 3, 2008·Cancer Research·Seong Muk KimSin-Soo Jeun
Jan 15, 2009·Liver International : Official Journal of the International Association for the Study of the Liver·Yongmin YanFei Mao
Mar 30, 2010·Stem Cells and Development·Masumi NaganoOsamu Ohneda
Sep 16, 2010·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Gaoxing LuoJun Wu
Nov 13, 2010·Journal of the Neurological Sciences·Jae-Hoon LeeHwi-Yool Kim
Apr 13, 2011·Neurology India·S K Shankar
May 14, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Alexander R MoschenHerbert Tilg
Jun 7, 2011·The American Journal of the Medical Sciences·Ling-Ling ShiDe-Wen Wang
Jul 5, 2011·Critical Care Medicine·Elisa R ZanierMaria-Grazia De Simoni
Jul 8, 2011·Stem Cells and Development·Marijke W MaijenburgCarlijn Voermans
Aug 17, 2011·Respiratory Research·Eun Sun KimWon Soon Park
Sep 24, 2011·Stem Cell Research & Therapy·Jung Yeon LimSin-Soo Jeun
Nov 10, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Eun Ju LeeHyo-Soo Kim
Nov 11, 2011·American Journal of Obstetrics and Gynecology·Andreina SchoeberleinDaniel V Surbek
Citations
Aug 31, 2013·PloS One·Wen-Wen LiTeng-Nan Lin
Nov 6, 2012·International Immunopharmacology·Yang LiJian Suo
Jan 11, 2014·Biomaterials·Cihui YanDongsheng Xiong
Nov 6, 2013·Journal of Zhejiang University. Science. B·Xiao-zhou MouCharlie Xiang
Jul 4, 2015·Cytotherapy·Bruna Maria ManziniÂngela Cristina Malheiros Luzo
Sep 1, 2015·Current Transplantation Reports·Jennifer R FerrerJason A Wertheim
Apr 14, 2015·Hepatobiliary & Pancreatic Diseases International : HBPD INT·Jin-Feng YangLan-Juan Li
Feb 16, 2016·Scientific Reports·Olfat A HammamAiman S El-Khatib
Sep 13, 2016·Scientific Reports·Giuseppe PettinatoXuejun Wen
Apr 26, 2013·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Dong-Liang LiJing-Jing Fan
Apr 11, 2017·Journal of Molecular Histology·Tingting ZhouJunqing Ma
Jun 11, 2020·Experimental and Therapeutic Medicine·Junfeng WangBingrong Zheng
Jan 27, 2016·Experimental Biology and Medicine·Hoda E MohamedAmal Mh Ghanim
Jun 7, 2017·Stem Cell Research & Therapy·Jie WangLanjuan Li
Nov 10, 2017·Scientific Reports·Ajay KumarShalmoli Bhattacharyya
Aug 26, 2018·Stem Cell Research & Therapy·Yong-Hong WangHong Tang
Aug 14, 2019·European Journal of Pharmacology·Piotr CzekajRoberto Gramignoli