In vivo hepatic differentiation of mesenchymal stem cells from human umbilical cord blood after transplantation into mice with liver injury

Biochemical and Biophysical Research Communications
Jiong YuLanjuan Li

Abstract

The aim of this study was to analyze the hepatic differentiation potential of human umbilical cord blood-derived mesenchymal stem cells (hUCBMSCs) after transplantation into severe combined immune deficiency (SCID) mice with liver injury induced by D-galactosamine/lipopolysaccharide (GalN/LPS) and to explore the possibility that cells can partially repair GalN/LPS-induced hepatic damage. Mononuclear cells (MNCs) were isolated from fresh human umbilical cord blood, characterized by flow cytometry, and then transplanted into GalN/LPS-injured mice. Specimens were collected at 7, 14, 21, and 28 days after hUCBMSC transplantation. Histopathological changes were analyzed by hematoxylin and eosin staining. Polymerase chain reaction (PCR) for a specific marker of human cells, the human Alu sequence, was performed to locate exogenous hUCBMSCs in mouse livers. Expression of human hepatocyte-specific markers such as human albumin (hALB), human alpha-fetoprotein (hAFP), human cytokeratin 18 (hCK18), and human cytokeratin 19 (hCK19) were analyzed by reverse transcriptase (RT)-PCR and immunohistochemical staining. The hUCBMSCs were positive for the human MSC-specific markers CD271, CD29, CD90, CD105, and CD73, but negative for CD31, CD79b, C...Continue Reading

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Citations

Nov 6, 2013·Journal of Zhejiang University. Science. B·Xiao-zhou MouCharlie Xiang
Sep 1, 2015·Current Transplantation Reports·Jennifer R FerrerJason A Wertheim
Apr 14, 2015·Hepatobiliary & Pancreatic Diseases International : HBPD INT·Jin-Feng YangLan-Juan Li
Apr 26, 2013·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Dong-Liang LiJing-Jing Fan
Apr 11, 2017·Journal of Molecular Histology·Tingting ZhouJunqing Ma
Jun 11, 2020·Experimental and Therapeutic Medicine·Junfeng WangBingrong Zheng
Jan 27, 2016·Experimental Biology and Medicine·Hoda E MohamedAmal Mh Ghanim
Aug 26, 2018·Stem Cell Research & Therapy·Yong-Hong WangHong Tang

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