In vivo high-resolution 3D overhauser-enhanced MRI in mice at 0.2 T

Contrast Media & Molecular Imaging
Philippe MassotEric Thiaudière

Abstract

Overhauser-enhanced MRI (OMRI) offers the potentiality of detecting low-concentrated species generated by specific biological processes. However molecular imaging applications of OMRI need significant improvement in spatial localization. Here it is shown that 3D-OMRI of a free radical injected in tumor-bearing mice can be performed at high anatomical resolution at a constant field. A 30 mm cavity operating at 5.43 GHz was inserted in a C-shaped magnet for proton MRI at 0.194 T. Nude mice with or without brain-implanted C6 rat glioma were positioned in the cavity and injected with TOPCA (1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrole-3-carboxylic acid). OMRI was performed in 3D within several minutes in the brain region without high overheating of the animals. Voxel size was 0.5 × 0.5 × 1 mm³ , providing good delineation of brain regions. Signal amplifications ranged from 2 in tumors to 10 in vessels several minutes after TOPCA injection. Time-course of signal enhancement could be measured by 2D OMRI at 15 s time intervals in a localized thin slice. The method opens the way for molecular imaging of biological activities able to generate OMRI-visible free radicals.

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Citations

Feb 28, 2016·Journal of Magnetic Resonance·Enrico RaveraGiacomo Parigi
Aug 14, 2012·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Gérard AudranGermain Obame
Mar 12, 2013·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Mathieu SarracanieMatthew S Rosen
Apr 15, 2014·Contrast Media & Molecular Imaging·Neha KoonjooPhilippe Mellet
Aug 28, 2013·Free Radical Biology & Medicine·Rheal A TownerHugo C Castro Faria-Neto
Dec 18, 2013·Organic & Biomolecular Chemistry·Gérard AudranEric Thiaudière
May 13, 2014·Chemical Communications : Chem Comm·Gérard AudranSylvain R A Marque
Jun 18, 2014·Molecular Pharmaceutics·Damien MonceletPhilippe Mellet
Apr 27, 2017·Nature Communications·David E J WaddingtonMatthew S Rosen
Oct 19, 2017·Antioxidants & Redox Signaling·Sergey I DikalovIgor Kirilyuk
Mar 2, 2018·NMR in Biomedicine·David E J WaddingtonMatthew S Rosen

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