PMID: 9420883Nov 1, 1995Paper

In vivo hormonal regulation of insulin-like growth factor binding protein-5 mRNA in the immature rat ovary

Journal of the Society for Gynecologic Investigation
L T PutowskiR M Rohan

Abstract

Despite the potential importance of insulin-like growth factor binding protein-5 (IGFBP-5) to follicular development, the hormonal regulation of this antigonadotropic IGFBP has not been investigated. Therefore, it was the objective of this study to eludicate the role of gonadotropins and estrogen in the in vivo regulation of IGFBP-5 mRNA expression. Two models of follicular development in immature rats were used. Specifically, rats were hypophysectomized and treated with FSH and/or diethylstilbestrol (DES). Alternatively, terminal follicular development was induced in intact immature rats by pregnant mare serum gonadotropin (PMSG) and hCG. The IGFBP-5 mRNA in whole ovarian RNA was assayed by Northern blot hybridization. Localization of expression in PMSG and hCG-stimulated ovaries was further assessed by in situ hybridization. Expression of IGFBP-5 mRNA was increased in ovaries from hypophysectomized rats. Treatment with FSH and/or DES did not alter the abundance of this mRNA. Treatment with PMSG induced a transient increase in IGFBP-5 expression that was localized in a subset of alpha-inhibin-negative follicles. At later times after PMSG, IGFBP-5 expression persisted in the surface epithelium but was not detected in large preo...Continue Reading

Citations

Jun 1, 1996·The International Journal of Biochemistry & Cell Biology·K M KelleyR G Rosenfeld
Apr 8, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alexandre BoyerDerek Boerboom

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