In vivo hydrolysis of S-adenosylmethionine induces the met regulon of Escherichia coli

Microbiology
Bernadette L LaMonte, Jeffrey A Hughes

Abstract

Regulation of methionine biosynthesis in Escherichia coli involves a complex of the MetJ aporepressor protein and S-adenosylmethionine (SAM) repressing expression of most genes in the met regulon. To test the role of SAM in the regulation of met genes directly, SAM pools were depleted by the in vivo expression of the cloned plasmid vector-based coliphage T3 SAM hydrolase (SAMase) gene. Cultures with in vivo SAMase activity were assayed for expression of the metA, B, C, E, F, H, J, K and R genes in cells grown in methionine-rich complete media as well as in defined media with and without l-methionine. In vivo SAMase activity dramatically induced expression between 11- and nearly 1000-fold depending on the gene assayed for all but metJ and metH, and these genes were induced over twofold. metJ : : Tn5 (aporepressor defective) and metK : : Tn5 (SAM synthetase impaired; produces <5 % of wild-type SAM) strains containing in vivo SAMase activity produced even higher met gene activity than that seen in comparably prepared cells with wild-type genes for all but metJ in a MetJ-deficient background. The SAMase-mediated hyperinduction of metH in wild-type cells and of the met genes assayed in metJ : : Tn5 and metK : : Tn5 cells provokes qu...Continue Reading

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Citations

Sep 28, 2012·MBio·Ryan W BogardJohn J Mekalanos
Jun 19, 2013·Annual Review of Microbiology·Michael J GrayUrsula Jakob
Dec 23, 2011·Journal of Applied Microbiology·G GölzT Alter
Oct 15, 2013·Environmental Microbiology·Pavel S NovichkovDmitry A Rodionov
May 29, 2018·Nature Ecology & Evolution·Jon Jerlström HultqvistDan I Andersson

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