In vivo identification of nuclear factors interacting with the conserved elements of box C/D small nucleolar RNAs.

Molecular and Cellular Biology
E CaffarelliI Bozzoni

Abstract

The U16 small nucleolar RNA (snoRNA) is encoded by the third intron of the L1 (L4, according to the novel nomenclature) ribosomal protein gene of Xenopus laevis and originates from processing of the pre-mRNA in which it resides. The U16 snoRNA belongs to the box C/D snoRNA family, whose members are known to assemble in ribonucleoprotein particles (snoRNPs) containing the protein fibrillarin. We have utilized U16 snoRNA in order to characterize the factors that interact with the conserved elements common to the other members of the box C/D class. In this study, we have analyzed the in vivo assembly of U16 snoRNP particles in X. laevis oocytes and identified the proteins which interact with the RNA by label transfer after UV cross-linking. This analysis revealed two proteins, of 40- and 68-kDa apparent molecular size, which require intact boxes C and D together with the conserved 5',3'-terminal stem for binding. Immunoprecipitation experiments showed that the p40 protein corresponds to fibrillarin, indicating that this protein is intimately associated with the RNA. We propose that fibrillarin and p68 represent the RNA-binding factors common to box C/D snoRNPs and that both proteins are essential for the assembly of snoRNP particl...Continue Reading

References

Jun 1, 1992·Genes & Development·S J BasergaX W Yang
Nov 11, 1987·Nucleic Acids Research·J F MilliganO C Uhlenbeck
May 1, 1994·Molecular and Cellular Biology·E CaffarelliI Bozzoni
Jan 1, 1995·Annual Review of Biochemistry·E S Maxwell, M J Fournier
Jul 1, 1995·Trends in Biochemical Sciences·J P BachellerieM J Fournier
Sep 1, 1994·Molecular and Cellular Biology·M NicolosoJ P Bachellerie
May 13, 1994·Science·M P Terns, J E Dahlberg
Apr 1, 1993·Trends in Biochemical Sciences·M J Fournier, E S Maxwell
Mar 1, 1996·European Journal of Biochemistry·R PeekW J Van Venrooij
Apr 17, 1997·Biochemical and Biophysical Research Communications·E CaffarelliI Bozzoni
Jun 1, 1997·Current Opinion in Cell Biology·D Tollervey, T Kiss
May 30, 1997·Cell·C M Smith, J A Steitz

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Citations

Oct 29, 2002·Chemistry & Biology·Lara B Weinstein SzewczakJoan A Steitz
Jul 31, 2014·Nucleic Acids Research·Gabrielle Deschamps-FrancoeurMichelle S Scott
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·A MichienziJ J Rossi
Jul 18, 2002·Gene·Irina K GogolevskayaDmitri A Kramerov
Nov 25, 2003·The EMBO Journal·Luisa Di StefanoKristian Helin
Jul 9, 1999·Molecular Biology of the Cell·A NarayananM P Terns
Oct 8, 1998·Molecular Biology of the Cell·T S LangeS A Gerbi
Dec 8, 1998·The EMBO Journal·A HenrasM Caizergues-Ferrer
Apr 16, 2003·Molecular Microbiology·Arina D OmerPatrick P Dennis
Nov 18, 2009·Molecular and Cellular Biology·Jingping GePhilip J Mason
Aug 8, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Udesh de SilvaBernard A Brown
Jun 6, 2002·Trends in Genetics : TIG·Alexander E Vinogradov

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