In vivo imaging and analysis of cerebrovascular hemodynamic responses and tissue oxygenation in the mouse brain

Nature Protocols
Kassandra KislerBerislav V Zlokovic

Abstract

Cerebrovascular dysfunction has an important role in the pathogenesis of multiple brain disorders. Measurement of hemodynamic responses in vivo can be challenging, particularly as techniques are often not described in sufficient detail and vary between laboratories. We present a set of standardized in vivo protocols that describe high-resolution two-photon microscopy and intrinsic optical signal (IOS) imaging to evaluate capillary and arteriolar responses to a stimulus, regional hemodynamic responses, and oxygen delivery to the brain. The protocol also describes how to measure intrinsic NADH fluorescence to understand how blood O2 supply meets the metabolic demands of activated brain tissue, and to perform resting-state absolute oxygen partial pressure (pO2) measurements of brain tissue. These methods can detect cerebrovascular changes at far higher resolution than MRI techniques, although the optical nature of these techniques limits their achievable imaging depths. Each individual procedure requires 1-2 h to complete, with two to three procedures typically performed per animal at a time. These protocols are broadly applicable in studies of cerebrovascular function in healthy and diseased brain in any of the existing mouse mod...Continue Reading

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Citations

Sep 3, 2019·Frontiers in Neurology·Şefik Evren Erdener, Turgay Dalkara
Oct 20, 2018·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Kazuto Masamoto, Alberto Vazquez
Nov 12, 2019·Cardiovascular Research·Christian StaehrVladimir V Matchkov
Oct 16, 2018·Biomedical Optics Express·Nami DavoodzadehGuillermo Aguilar
Jun 27, 2019·Lasers in Surgery and Medicine·Nami DavoodzadehGuillermo Aguilar
Jul 19, 2019·Frontiers in Neuroscience·Emmanuelle ChaigneauSerge Charpak
May 16, 2020·Frontiers in Aging Neuroscience·Amy R NelsonBerislav V Zlokovic
Mar 3, 2020·Frontiers in Cellular Neuroscience·Kassandra KislerBerislav V Zlokovic
Feb 14, 2019·Archives of Pharmacal Research·Jin-Hui Yoon, Yong Jeong
Jun 27, 2019·Nature Neuroscience·Angeliki M NikolakopoulouBerislav V Zlokovic
Feb 18, 2021·European Journal of Nuclear Medicine and Molecular Imaging·Daniel RazanskyRuiqing Ni
May 30, 2019·The Journal of Physical Chemistry Letters·Andrew T FrancisDan Fu

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