In vivo imaging of injured cortical axons reveals a rapid onset form of Wallerian degeneration.

BMC Biology
Alison J CantyVincenzo De Paola

Abstract

Despite the widespread occurrence of axon and synaptic loss in the injured and diseased nervous system, the cellular and molecular mechanisms of these key degenerative processes remain incompletely understood. Wallerian degeneration (WD) is a tightly regulated form of axon loss after injury, which has been intensively studied in large myelinated fibre tracts of the spinal cord, optic nerve and peripheral nervous system (PNS). Fewer studies, however, have focused on WD in the complex neuronal circuits of the mammalian brain, and these were mainly based on conventional endpoint histological methods. Post-mortem analysis, however, cannot capture the exact sequence of events nor can it evaluate the influence of elaborated arborisation and synaptic architecture on the degeneration process, due to the non-synchronous and variable nature of WD across individual axons. To gain a comprehensive picture of the spatiotemporal dynamics and synaptic mechanisms of WD in the nervous system, we identify the factors that regulate WD within the mouse cerebral cortex. We combined single-axon-resolution multiphoton imaging with laser microsurgery through a cranial window and a fluorescent membrane reporter. Longitudinal imaging of > 150 individuall...Continue Reading

References

Jul 1, 1984·The Journal of Cell Biology·R J LasekS T Brady
Feb 11, 2000·Annals of the New York Academy of Sciences·L GuthO Steward
Aug 3, 2001·The Journal of Physiology·T H Gillingwater, R R Ribchester
Jul 12, 2002·Microscopy Research and Technique·Kazuho Hirata, Masaru Kawabuchi
Sep 11, 2002·Trends in Neurosciences·Michael P Coleman, V Hugh Perry
Feb 15, 1963·Proceedings of the National Academy of Sciences of the United States of America·R L FRIEDE
Feb 20, 2004·Current Biology : CB·Ali SajadiPatrick Aebischer
Apr 23, 2004·Journal of Neuroscience Methods·Bogdan BeirowskiMichael P Coleman
Nov 16, 2004·Neuron·Derron L BishopJeff W Lichtman
Dec 17, 2004·Nature·Mehmet Fatih YanikAdela Ben-Yakar
Apr 12, 2005·Nature Medicine·Martin KerschensteinerThomas Misgeld
Jul 27, 2005·The Journal of Cell Biology·Jing WangZhigang He
Oct 15, 2005·Nature Reviews. Neuroscience·Michael Coleman
May 23, 2006·Nature Reviews. Neuroscience·Thomas Misgeld, Martin Kerschensteiner
Jun 2, 2006·Brain : a Journal of Neurology·Thomas H GillingwaterRichard R Ribchester
Apr 5, 2007·Nature Protocols·Nadine GogollaPico Caroni
May 18, 2007·Annual Review of Neuroscience·Mauricio E Vargas, Ben A Barres
Aug 24, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ali ErtürkFrank Bradke
Sep 8, 2007·Progress in Neurobiology·Smita Saxena, Pico Caroni
Jan 16, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Wakoto MatsudaTakeshi Kaneko
May 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Cyril DrayFranck Debarbieux
Jul 21, 2009·Nature Protocols·Anthony HoltmaatLinda Wilbrecht
Feb 4, 2010·PLoS Biology·Jonathan Gilley, Michael P Coleman
Mar 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Johanna KnöferlePaul Lingor
Mar 30, 2010·Annual Review of Neuroscience·Michael P Coleman, Marc R Freeman
Aug 21, 2010·Frontiers in Neuroenergetics·Anna Letizia Allegra MascaroFrancesco S Pavone
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Marcel OberlaenderChristiaan P J de Kock
Mar 25, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Alessandro Di MaioYoung-Jin Son
Jan 11, 2012·The Journal of Cell Biology·Jack T WangBen A Barres
Jan 24, 2012·Nature Methods·Matthew J FarrarChris B Schaffer
Mar 17, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Allison F RosenbergMichael Granato

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