In vivo imaging of lymphatic drainage of cerebrospinal fluid in mouse

Fluids and Barriers of the CNS
Emily MathieuYeni H Yücel

Abstract

Mouse models are commonly used to study central nervous system disorders, in which cerebrospinal fluid (CSF) drainage may be disturbed. However, mouse CSF drainage into lymphatics has not been thoroughly characterized. We aimed to image this using an in vivo approach that combined quantum dot fluorescent nanoparticles with hyperspectral imaging. Quantum dot 655 was injected into the CSF of the cisterna magna in seven mice and visualized by in vivo hyperspectral imaging at time points 20 and 40 min, 1, 2, and 6 h after injection. In controls (n = 4), quantum dots were applied directly onto intact dura mater covering the cisterna magna. After imaging, lymph nodes in the neck were harvested and processed post-mortem for histological analysis. After injection into the CSF, quantum dot signal was detected in vivo in submandibular lymph nodes of all mice studied as early as 20 min, but not in controls. Post-mortem gross and histological examination of lymph nodes confirmed in vivo observations. Non-invasive in vivo hyperspectral imaging is a useful tool to study CSF lymphatic drainage and is relevant to understanding this pathway in CNS disease models.

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Citations

Jul 17, 2015·Neurosurgery·R Loch Macdonald
Mar 18, 2015·Chemical Society Reviews·K David Wegner, Niko Hildebrandt
Nov 16, 2014·Neuroradiology·Long ChenKieran Murphy
Jan 28, 2017·Clinical & Experimental Ophthalmology·Peter WostynPeter Paul De Deyn
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Mar 30, 2017·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Eugene V GolanovGavin W Britz
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Jan 8, 2019·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Satoshi TsutsumiYukimasa Yasumoto
Dec 14, 2018·Investigative Ophthalmology & Visual Science·Emily MathieuYeni H Yücel
Mar 28, 2020·Science Translational Medicine·Xiaowei WangMaiken Nedergaard
Jan 20, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Lingxiao ZhangDaishun Ling
Jun 24, 2021·Journal of Neurochemistry·Jiru ZhouHua Feng
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Sep 28, 2021·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Aria R HendersonEsak Lee
Mar 12, 2018··Eva M. Sevick-MuracaFred Velasquez

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Methods Mentioned

BETA
imaging technique
fluorescence imaging
imaging techniques

Software Mentioned

Maestro

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