In Vivo Imaging of Retinal Oxidative Stress Using a Reactive Oxygen Species-Activated Fluorescent Probe

Investigative Ophthalmology & Visual Science
Megan C PruntyMachelle T Pardue

Abstract

In vivo methods for detecting oxidative stress in the eye would improve screening and monitoring of the leading causes of blindness: diabetic retinopathy, glaucoma, and age-related macular degeneration. To develop an in vivo biomarker for oxidative stress in the eye, we tested the efficacy of a reactive oxygen species (ROS)-activated, near-infrared hydrocyanine-800CW (H-800CW) fluorescent probe in light-induced retinal degeneration (LIRD) mouse models. After intravitreal delivery in LIRD rats, fluorescent microscopy was used to confirm that the oxidized H-800CW appeared in the same retinal layers as an established ROS marker (dichlorofluorescein). Dose-response curves of increasing concentrations of intravenously injected H-800CW demonstrated linear increases in both intensity and total area of fundus hyperfluorescence in LIRD mice, as detected by scanning laser ophthalmoscopy. Fundus hyperfluorescence also correlated with the duration of light damage and functional deficits in vision after LIRD. In LIRD rats with intravitreal injections of H-800CW, fluorescent labeling was localized to photoreceptor inner segments, similar to dichlorofluorescein. Hydrocyanine-800CW detects retinal ROS in vivo and shows potential as a novel bio...Continue Reading

Citations

Jan 13, 2018·Antioxidants & Redox Signaling·Xiqian JiangJin Wang
Oct 28, 2017·Bioscience Reports·Qiaoyun Gong, Guanfang Su
Sep 11, 2018·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Seung-Jun SeoJong-Ki Kim
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Feb 21, 2021·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Yingmin WangXingyou Ma
Feb 28, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Junying LuShu Nie
Nov 15, 2020·Progress in Retinal and Eye Research·Deoye Tonade, Timothy S Kern

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