In vivo imaging of the tonoplast intrinsic protein family in Arabidopsis roots.

BMC Plant Biology
Stefano GattolinLorenzo Frigerio

Abstract

Tonoplast intrinsic proteins (TIPs) are widely used as markers for vacuolar compartments in higher plants. Ten TIP isoforms are encoded by the Arabidopsis genome. For several isoforms, the tissue and cell specific pattern of expression are not known. We generated fluorescent protein fusions to the genomic sequences of all members of the Arabidopsis TIP family whose expression is predicted to occur in root tissues (TIP1;1 and 1;2; TIP2;1, 2;2 and 2;3; TIP4;1) and expressed these fusions, both individually and in selected pairwise combinations, in transgenic Arabidopsis. Analysis by confocal microscopy revealed that TIP distribution varied between different cell layers within the root axis, with extensive co-expression of some TIPs and more restricted expression patterns for other isoforms. TIP isoforms whose expression overlapped appeared to localise to the tonoplast of the central vacuole, vacuolar bulbs and smaller, uncharacterised structures. We have produced a comprehensive atlas of TIP expression in Arabidopsis roots, which reveals novel expression patterns for not previously studied TIPs.

References

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Citations

Jun 20, 2014·Plant Physiology and Biochemistry : PPB·Galina V NovikovaNatalie Obroucheva
Aug 7, 2014·Functional & Integrative Genomics·Preetom RegonSanjib Kumar Panda
Dec 19, 2013·Journal of Proteome Research·Arnoud J GroenKathryn S Lilley
Apr 12, 2011·Plant, Cell & Environment·Adriana M Almeida-RodriguezJoan Laur
Dec 18, 2012·The FEBS Journal·Li XiangWim Van den Ende
Feb 22, 2013·Traffic·Doan-Trung Luu, Christophe Maurel
Nov 2, 2014·Journal of Integrative Plant Biology·Yumi HirakawaTakumi Higaki
Mar 23, 2011·Plant Science : an International Journal of Experimental Plant Biology·Claus-Peter Witte
Mar 13, 2015·Plant Signaling & Behavior·Ling BaiChun-Peng Song
Jan 19, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Bart Van GansbekeDiane E Mather
Apr 16, 2019·Plant, Cell & Environment·Steven FootittLorenzo Frigerio
Jun 1, 2019·Plant, Cell & Environment·Yun HuangYuan-Yue Shen
Sep 4, 2015·Physiological Reviews·Christophe MaurelLionel Verdoucq
May 25, 2010·Biochemical Society Transactions·Stefano GattolinLorenzo Frigerio
Jan 29, 2019·Frontiers in Plant Science·Fabrizio BarozziGian-Pietro Di Sansebastiano
Jan 13, 2019·International Journal of Molecular Sciences·Zhi-Juan FengYa-Ming Gong
May 28, 2020·Frontiers in Plant Science·Sabrina Kaiser, David Scheuring
Oct 18, 2019·Plant Molecular Biology·Mst Hur MadinaHugo Germain
Feb 23, 2020·Plant Physiology and Biochemistry : PPB·Roshan Kumar SinghManoj Prasad

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Methods Mentioned

BETA
transgenic
confocal microscopy

Software Mentioned

Mimics
Leica LAS - AF Lite

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