In vivo imaging of unstained tissues using a compact and flexible multiphoton microendoscope.

Journal of Biomedical Optics
Christopher M BrownChris Xu

Abstract

We use a compact and flexible multiphoton microendoscope (MPME) to acquire in vivo images of unstained liver, kidney, and colon from an anesthetized rat. The device delivers femtosecond pulsed 800 nm light from the core of a raster-scanned dual-clad fiber (DCF), which is focused by a miniaturized gradient-index lens assembly into tissue. Intrinsic fluorescence and second-harmonic generation signal from the tissue is epi-collected through the core and inner clad of the same DCF. The MPME has a rigid distal tip of 3 mm in outer diameter and 4 cm in length. The image field-of-view measures 115 μm by 115 μm and was acquired at 4.1 frames/s with 75 mW illumination power at the sample. Organs were imaged after anesthetizing Sprague-Dawley rats with isofluorane gas, accessing tissues via a ventral-midline abdominal incision, and isolating the organs with tongue depressors. In vivo multiphoton images acquired from liver, kidney, and colon using this device show features similar to that of conventional histology slides, without motion artifact, in ~75% of imaged frames. To the best of our knowledge, this is the first demonstration of multiphoton imaging of unstained tissue from a live subject using a compact and flexible MPME device.

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Citations

Dec 18, 2015·Scientific Reports·Guillaume DucourthialFrédéric Louradour
Jun 29, 2016·Pflügers Archiv : European journal of physiology·Ina Maria Schießl, Hayo Castrop
Jul 12, 2016·Laboratory Investigation; a Journal of Technical Methods and Pathology·Sarah KretschmerPeter König
Feb 24, 2016·PloS One·Fatemeh NooshabadiKristen C Maitland
Feb 8, 2018·Lasers in Medical Science·Kexin HeZhining Fan
Oct 11, 2016·Journal of Biomedical Optics·Esben Ravn AndresenHervé Rigneault
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Oct 10, 2013·Optics Letters·Dimitre G OuzounovChris Xu
Jan 24, 2018·Biomedical Optics Express·C H HageF Louradour
May 15, 2013·Biomedical Optics Express·David M HulandChris Xu
Jul 9, 2014·Journal of Biomedical Optics·Claire LefortDarine Abi Haidar
Jul 13, 2013·Biomedical Optics Express·Riccardo CicchiFrancesco S Pavone
Jan 24, 2018·Biomedical Optics Express·David M SmallNozomi Nishimura
May 28, 2016·Biomedical Optics Express·N V KuzminM L Groot
May 21, 2019·Journal of Clinical Gastroenterology·Ben GloverNisha Patel
Mar 25, 2020·Journal of Neuroradiology. Journal De Neuroradiologie·Sylvia M BardetAymeric Rouchaud
Nov 16, 2021·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Vytautas KučikasMarc A M J van Zandvoort

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