In vivo imaging of unstained tissues using long gradient index lens multiphoton endoscopic systems.

Biomedical Optics Express
David M HulandChris Xu

Abstract

We characterize long (up to 285 mm) gradient index (GRIN) lens endoscope systems for multiphoton imaging. We fabricate a portable, rigid endoscope system suitable for imaging unstained tissues, potentially deep within the body, using a GRIN lens system of 1 mm diameter and 8 cm length. The portable device is capable of imaging a ~200 µm diameter field of view at 4 frames/s. The lateral and axial resolution in water is 0.85 µm and 7.4 µm respectively. In vivo images of unstained tissues in live, anesthetized rats using the portable device are presented. These results show great promise for GRIN endoscopy to be used clinically.

References

Apr 6, 1990·Science·W DenkW W Webb
Mar 14, 2002·Lasers in Surgery and Medicine·Eric J Seibel, Quinn Y J Smithwick
Jun 21, 2003·Optics Letters·Juergen C Jung, Mark J Schnitzer
Dec 12, 2003·Journal of Neurophysiology·Michael J LeveneWatt W Webb
May 7, 2004·Journal of Neurophysiology·Juergen C JungMark J Schnitzer
Aug 31, 2004·Lasers in Surgery and Medicine·Petra Wilder-SmithTatiana Krasieva
Sep 30, 2005·Optics Letters·Benjamin A FlusbergMark J Schnitzer
Apr 20, 2006·Journal of Neuroscience Methods·Quoc-Thang NguyenDavid Kleinfeld
Apr 22, 2006·Optics Letters·Mon Thiri MyaingXingde Li
Mar 30, 2007·Microscopy Research and Technique·K KönigM Kaatz
Sep 18, 2007·Journal of Biomedical Optics·Ling FuMin Gu
Apr 11, 2009·Proceedings - Society of Photo-Optical Instrumentation Engineers·Sushmita MukherjeeDouglas S Scherr
Apr 17, 2009·Optics Letters·Michael E DurstChris Xu
Aug 6, 2009·Optics Express·Demirhan KobatChris Xu
Sep 4, 2009·Journal of Biomedical Optics·Chun-Chin WangChen-Yuan Dong
Jan 25, 2011·Biomedical Optics Express·Johanna M Dela CruzWarren R Zipfel
Apr 20, 2011·Optics Express·Rajesh S PillaiDavid D Sampson
Oct 19, 2011·Proceedings of the National Academy of Sciences of the United States of America·David R RiveraChris Xu
Oct 25, 2011·The Journal of Urology·Ranjith RamasamyPeter N Schlegel
Oct 28, 2011·Journal of Biomedical Optics·Demirhan KobatChris Xu
Apr 17, 2012·Journal of Biomedical Optics·Ina PavlovaWatt W Webb
Apr 20, 2012·Optics Letters·David R RiveraChris Xu
May 9, 2012·Journal of Biomedical Optics·Christopher M BrownChris Xu

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Citations

Jul 13, 2013·Journal of Pediatric Surgery·Amit AggarwalOliver J Muensterer
Mar 19, 2013·Journal of Healthcare Engineering·Supang Khondee, Thomas D Wang
Aug 27, 2015·Biomedical Optics Express·Xiyu DuanThomas D Wang
Oct 10, 2013·Optics Express·Salma FarahiChristophe Moser
Feb 3, 2016·Applied Optics·Ryszard BuczynskiMohammad R Taghizadeh
May 29, 2013·Scientific Reports·Asaf ShahmoonZeev Zalevsky
Jul 6, 2012·Journal of Biophotonics·Natalia GladkovaFelix Feldchtein
May 15, 2013·Biomedical Optics Express·David M HulandChris Xu
Jun 2, 2015·Archives of Pathology & Laboratory Medicine·Manu JainSushmita Mukherjee
May 28, 2016·Biomedical Optics Express·N V KuzminM L Groot
Jun 15, 2016·Lasers in Medical Science·Lianhuang LiJianxin Chen
Nov 18, 2015·BJU International·Manu JainSushmita Mukherjee
Jul 12, 2016·Laboratory Investigation; a Journal of Technical Methods and Pathology·Sarah KretschmerPeter König
Jul 22, 2017·International Wound Journal·Jessica E S SutcliffeDavid L Becker
Dec 24, 2018·Journal of Biomedical Optics·Lin HuangShuo Tang
Oct 12, 2019·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·M Rocio Servin-VencesAnca Margineanu
Sep 21, 2019·Nature Communications·Chao HeMartin J Booth
Nov 22, 2014·Journal of Biomedical Optics·David M HulandAshutosh K Tewari
Mar 3, 2020·Frontiers in Immunology·Nathaniel H Allan-RahillDavid M Small
May 16, 2018·Biomedical Optics Express·Farhad AkhoundiKhanh Kieu
Apr 25, 2019·Advanced Science·Ashwathama DilipkumarSebastian Schürmann

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Methods Mentioned

BETA
biopsies
biopsy
sedation

Software Mentioned

MPScan

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