In vivo imaging of xenograft tumors using an epidermal growth factor receptor-specific affibody molecule labeled with a near-infrared fluorophore.

Neoplasia : an International Journal for Oncology Research
Haibiao GongDavid Michael Olive

Abstract

Overexpression of epidermal growth factor receptor (EGFR) is associated with many types of cancers. It is of great interest to noninvasively image the EGFR expression in vivo. In this study, we labeled an EGFR-specific Affibody molecule (Eaff) with a near-infrared (NIR) dye IRDye800CW maleimide and tested the binding of this labeled molecule (Eaff800) in cell culture and xenograft mouse tumor models. Unlike EGF, Eaff did not activate the EGFR signaling pathway. Results showed that Eaff800 was bound and taken up specifically by EGFR-overexpressing A431 cells. When Eaff800 was intravenously injected into nude mice bearing A431 xenograft tumors, the tumor could be identified 1 hour after injection and it became most prominent after 1 day. Images of dissected tissue sections demonstrated that the accumulation of Eaff800 was highest in the liver, followed by the tumor and kidney. Moreover, in combination with a human EGFR type 2 (HER2)-specific probe Haff682, Eaff800 could be used to distinguish between EGFR- and HER2-overexpressing tumors. Interestingly, the organ distribution pattern and the clearance rate of Eaff800 were different from those of Haff682. In conclusion, Eaff molecule labeled with a NIR fluorophore is a promising mo...Continue Reading

Citations

May 5, 2016·Oncotarget·Cristina MartelliLuisa Ottobrini
Apr 5, 2017·Annual Review of Analytical Chemistry·Xiaowen YuSylvia Daunert
Apr 23, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jiyoung Oh, James S Malter
Jan 26, 2017·Chemical Communications : Chem Comm·Yi WangMarcel P Bruchez
Jul 6, 2016·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Ana Luiza Ribeiro de SouzaBrian W Pogue
Mar 25, 2017·Experimental & Molecular Medicine·Fredrik Y Frejd, Kyu-Tae Kim
Dec 25, 2013·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Jingjuan QiaoJenny J Yang
Mar 8, 2014·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Ajay ShrivastavaMichael F Tweedle
Feb 22, 2012·Cancer Immunology, Immunotherapy : CII·Alessa PardoStefan Barth
Sep 5, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rahul PalAnand T N Kumar
Mar 16, 2017·ACS Nano·Beatriz PelazWolfgang J Parak

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