In vivo immunoadsorption of antipig antibodies in baboons using a specific Gal(alpha)1-3Gal column

Transplantation
S TaniguchiD K Cooper

Abstract

The major role of anti-alphaGal antibodies in the hyperacute rejection of pig organs by humans and baboons has been clearly demonstrated. Spacered alpha-galactose disaccharide (Gal(alpha1)-3Gal) hapten was produced by chemical synthesis and covalently attached to a flexible, hydrophilic polymer (PAA), which in turn was covalently coupled to macroporous glass beads, forming an immunoadsorbent that is mechanically and chemically stable and can be sterilized. The extracorporeal immunoadsorption (EIA) of anti-alphaGal antibodies using this column has been investigated in vivo in 3 baboons. In Baboon 1 (which had hyperacutely rejected a pig heart transplant 4 months previously, was not splenectomized, and did not receive any pharmacologic immunosuppression) the levels of anti-alphaGal antibody and antipig IgM and IgG, as well as serum cytotoxicity, fell significantly after each of 3 EIAs but were not eliminated. Serum cytotoxicity, antipig immunoglobulin and anti-alphaGal antibody rose steeply within 24 hr of the final EIA, suggesting that the return of cytotoxicity was associated with anti-alphaGa1 antibody. In Baboons 2 and 3 (which were immunologically naive and splenectomized, and received triple drug immunosuppressive therapy) ...Continue Reading

References

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Citations

Nov 27, 2002·Biotechnology and Bioengineering·Sujatha KaroorLee W Henderson
Feb 16, 2005·Transplant International : Official Journal of the European Society for Organ Transplantation·Angélique BrossayHervé Watier
Apr 1, 2004·The Surgical Clinics of North America·Ruediger Hoerbelt, Joren C Madsen
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Sep 19, 2003·Chest Surgery Clinics of North America·Thomas K Waddell, Mark D Peterson
Nov 15, 2000·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·U DoenzP Mohacsi
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Dec 22, 1999·Current Opinion in Chemical Biology·X ChenP G Wang
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Nov 15, 2006·Transplantation·Sandra CrikisAnthony J F d'Apice
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Apr 2, 2002·Journal of Cardiac Surgery·M S SandrinI F McKenzie
Mar 19, 2002·Xenotransplantation·Beverly J HolmesHugh F S Davies

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